{"id":1010,"date":"2023-07-13T15:44:56","date_gmt":"2023-07-13T15:44:56","guid":{"rendered":"https:\/\/sites.rutgers.edu\/edna\/?page_id=1010"},"modified":"2023-09-21T02:03:58","modified_gmt":"2023-09-21T02:03:58","slug":"standard-practices","status":"publish","type":"page","link":"https:\/\/sites.rutgers.edu\/edna\/standard-practices\/","title":{"rendered":"Standard Practices: Contamination, Cleaning, &amp; Controls"},"content":{"rendered":"<p style=\"text-align: center\"><span style=\"font-weight: 400\">eDNA is an incredibly sensitive technology<\/span><span style=\"font-weight: 400\">\u00a0\u2014 species detection assays can detect down to just 2-3 copies <\/span><span style=\"font-weight: 400\">of <\/span><span style=\"font-weight: 400\">target DNA per reaction.<\/span><span style=\"font-weight: 400\"> While this sensitivity <\/span><span style=\"font-weight: 400\">provides the ability to detect species<\/span><span style=\"font-weight: 400\"> at incredibly low abundance levels, it leaves eDNA samples<\/span><span style=\"font-weight: 400\"> vulnerable to contamination throughout the collection, extraction, and amplification process. Contamination occurs when DNA outside of the sampled medium is introduced to a sample. The introduction of<\/span><span style=\"font-weight: 400\">\u00a0<\/span><span style=\"font-weight: 400\">DNA from the target species from other sources can yield a false positive result for a given sample.<\/span><\/p>\n<p style=\"text-align: center\"><span style=\"font-weight: 400\">To prevent <\/span><span style=\"font-weight: 400\">contamination <\/span><span style=\"font-weight: 400\">into a sample<\/span><span style=\"font-weight: 400\">, all instruments and sampling equipment<\/span><span style=\"font-weight: 400\">\u00a0<\/span><span style=\"font-weight: 400\">that come into contact with samples <\/span><span style=\"font-weight: 400\">must be properly cleaned prior to<\/span><span style=\"font-weight: 400\"> use and between sampling efforts<\/span><span style=\"font-weight: 400\">. DNA can be removed <\/span><span style=\"font-weight: 400\">using a bleach solution, through flame sterilization, or via UV exposure.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: center\"><em>Below, you&#8217;ll find some of the methods we use to decontaminate our sampling and laboratory materials.\u00a0<\/em><\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>eDNA is an incredibly sensitive technology\u00a0\u2014 species detection assays can detect down to just 2-3 copies of target DNA per reaction. While this sensitivity provides the ability to detect species &hellip; <a href=\"https:\/\/sites.rutgers.edu\/edna\/standard-practices\/\" class=\"\">Read More<\/a><\/p>\n","protected":false},"author":2812,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-custom.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1010","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Standard Practices: Contamination, Cleaning, &amp; Controls - Environmental DNA Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.rutgers.edu\/edna\/standard-practices\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Standard Practices: Contamination, Cleaning, &amp; Controls - Environmental DNA Lab\" \/>\n<meta property=\"og:description\" content=\"eDNA is an incredibly sensitive technology\u00a0\u2014 species detection assays can detect down to just 2-3 copies of target DNA per reaction. 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While this sensitivity provides the ability to detect species &hellip; Read More","og_url":"https:\/\/sites.rutgers.edu\/edna\/standard-practices\/","og_site_name":"Environmental DNA Lab","article_modified_time":"2023-09-21T02:03:58+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/sites.rutgers.edu\/edna\/standard-practices\/","url":"https:\/\/sites.rutgers.edu\/edna\/standard-practices\/","name":"Standard Practices: Contamination, Cleaning, &amp; Controls - Environmental DNA Lab","isPartOf":{"@id":"https:\/\/sites.rutgers.edu\/edna\/#website"},"datePublished":"2023-07-13T15:44:56+00:00","dateModified":"2023-09-21T02:03:58+00:00","breadcrumb":{"@id":"https:\/\/sites.rutgers.edu\/edna\/standard-practices\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/sites.rutgers.edu\/edna\/standard-practices\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/sites.rutgers.edu\/edna\/standard-practices\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/sites.rutgers.edu\/edna\/"},{"@type":"ListItem","position":2,"name":"Standard Practices: Contamination, Cleaning, &amp; Controls"}]},{"@type":"WebSite","@id":"https:\/\/sites.rutgers.edu\/edna\/#website","url":"https:\/\/sites.rutgers.edu\/edna\/","name":"Environmental DNA Lab","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/sites.rutgers.edu\/edna\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/sites.rutgers.edu\/edna\/wp-json\/wp\/v2\/pages\/1010"}],"collection":[{"href":"https:\/\/sites.rutgers.edu\/edna\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.rutgers.edu\/edna\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.rutgers.edu\/edna\/wp-json\/wp\/v2\/users\/2812"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.rutgers.edu\/edna\/wp-json\/wp\/v2\/comments?post=1010"}],"version-history":[{"count":27,"href":"https:\/\/sites.rutgers.edu\/edna\/wp-json\/wp\/v2\/pages\/1010\/revisions"}],"predecessor-version":[{"id":1509,"href":"https:\/\/sites.rutgers.edu\/edna\/wp-json\/wp\/v2\/pages\/1010\/revisions\/1509"}],"wp:attachment":[{"href":"https:\/\/sites.rutgers.edu\/edna\/wp-json\/wp\/v2\/media?parent=1010"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}