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Nikolai Borysyuk, Assistant Research Professor

I have over 15 years experience of academic and applied innovative research in the area of molecular biology and biotechnology. During 1982-1989, after graduating from Kiev State University, I worked on the inheritance and molecular organization of rRNA genes in somatic hybrids getting a solid practice in plant molecular biology at the Moscow State University. In 1990 I was awarded with the prestigious A. von Humboldt Fellowship, which allowed me to continue my academic study on the evolution of ribosomal RNA genes and to initiate a practically-oriented project on the construction of plant high-copy-number transformation vectors at the University of Tübingen, Germany.

I joined the Institute of Plant Genetics, (Gatersleben) in 1992, where I organized a molecular biology group for studying gene expression during plant development, initiated and managed two international projects. In particular, I developed an effective method for the isolation of embryogenesis-related genes, cloned and characterized a number of genes including a plant chaperon, calreticulin, important for correct protein folding and secretion.

In 1996, I joined the Biotech Center of Rutgers University, working as a Phytomedics scientist in Dr. Raskin’s laboratory. Supervising a research group, I contributed to the development of new technologies for the production of recombinant proteins, based on the rhizosecretion from hydroponically grown plants. Rhizosecretion which offers a low-cost, large-scale recombinant protein production system became the basic technology of Phytomedics. In addition, I invented a method for the construction of a new plant transformation vector to significantly increase expression of valuable pharmaceutical proteins in transgenic plants.

Publications

  1. Komarnytsky, S., N. I. Borisjuk, N. Yakoby, A. Garvey and I. Raskin. Co-secretion of proteinase inhibitor enhances stability of antibodies produced by plant roots. Plant Physiol., 141: pp. 1185-1193, 2006.
  2. Komarnytsky, S., Gaume, A., Garvey, A., Borisjuk, N., Raskin, I. Quick and efficient system for antibiotic-free expression of heterologous genes in tobacco roots. Plant Cell Reports, 22: 765-773, 2004.
  3. Komarnytsky, S., Borisjuk, N. Functional analysis of promoter elements in plants. In: Genetic Engineering: Principles and Methods (Ed. J. K. Setlow), vol. 23, pp. 113-141, (2003).
  4. Volkov, R., Panchuk, I., Borisjuk, L., Borisjuk, N. Ribosomal DNA in plants: molecular organization, evolution and applications (Review). Cytology and Genetics, (Ukrainian, English summary), 1: 72-79, 2003.
  5. Gaume, A., Komarnytsky, S., Borisjuk, N., Raskin, I. Rhizosecretion of recombinant proteins from plant hairy roots. Plant Cell Reports, 21: 1188-1193, 2003.
  6. Ilya Raskin, David M. Ribnicky, Slavko Komarnytsky, Nebojša Ilić, Alexander Poulev, Nikolai Borisjuk, Anita Brinker, Diego A. Moreno, Christophe Ripoll, Nir Yakoby, Joseph M. O’Neal, Teresa Cornwell, Ira Pastor and Bertold Fridlender. Plants and human health in the twenty-first century. Trends in Biotechnology, 2002, 20:12:522-531.
  7. Borisjuk, N., Borisjuk, L., Komarnytsky, S., Timeva, S., Hemleben, V., Gleba, Y., Raskin, I. Tobacco ribosomal DNA spacer element stimulates amplification and expression of heterologous genes. Nature Biotechnology, 18: 1303-1306 (2000).
  8. Komarnytsky, S., Borisjuk, N., Borisjuk, L., Alam, M., Raskin, I. Production of recombinant proteins in the guttation fluid. Plant Physiology, 124: 1-7 (2000).
  9. Borisjuk, N., Borisjuk, L., Logendra, S., Petersen, F., Gleba, Y., Raskin, I. Production of recombinant proteins in plant root exudates. Nature Biotechnology, 17: 466-469 (1999).
  10. Gleba, D., Borisjuk, N., Borisjuk, L., Kneer, R., Poulev, A., Skarzhinskaya, M., Dushenkov, S., Logendra, S., Gleba, Y., Raskin, I. Radical approaches to phytoremediation and molecular farming. Proc. Nat. Acad. Sci. USA, 96: 5973-5977 (1999).
  11. Volkov, R., N. Borisjuk, I. Panchuk, D. Schweizer, and V. Hemleben. Elimination and rearrangement of parental rDNA in the allotetraploid Nicotiana
    tabacum    . Mol. Biol. Evol. 16: 311-320 (1999).
  12. Borisjuk, N., L. Sitailo, A. Tewes and R. Manteuffel. Defferential expression and sequence analysis of three ribosomal protein S17, L15 and S5 induced during the early stages of somatic embryogenesis in Nicotiana plumbaginifolia. Prepared for publication in Molec Gen Genet (1999).
  13. Borisjuk, N., L. Sitailo, L. Borisjuk, K. Adler, L. Malysheva, A. Tewes and R. Manteuffel. Calreticulin in plant cells: primary structure, developmentally regulated expression and intracellular distribution. Planta 206: 504-514 (1998).
  14. Borisjuk, N., Davidjuk, I., Kostyshin, S., Miroshnichenko, G., Velasko, R., Hemleben, V., Volkov, R. Structural analysis of rDNA in the genus Nicotiana. Plant Mol Biol 35, 655-660 (1997).
  15. Friesen, N., Borisjuk, N., Mes, T., Klaas, M., Hanelt, P. Allotetraploid origin of Allium altyncolicum (Alliaceae, Allium sect. Schoenoprasum) as investigated by kariological and molecular markers. Plant Systematics and Evolution 206, 317-335 (1997).
  16. Zanke, C., Borisjuk, N., Ruoss, B., Schilde-Rentschler, L., Ninnemann, H., Hemleben, V. A specific oligonucleotide of the 5S rDNA spacer and species-specific elements identify symmetric somatic hybrids between Solanum tuberosum and S. pinnatisectum. Theor. Appl. Genet. 90, 720-726 (1995).
  17. Stadler, M., Stelzer, T., Borisjuk, N., Zanke, C., Schilde-Rentschler, L., Hemleben, V. Distribution of novel and known repeated elements of Solanum and application for the identification of somatic hybrids among Solanum species. Theor. Appl. Genet., 91, 1271-1278 (1995).
  18. Borisjuk, N., Borisjuk, L., Petjuch, G., Hemleben, V. Comparison of nuclear ribosomal RNA genes among Solanum species and other Solanaceae. Genome 37, 271-279 (1994).
  19. Schweizer, G., Borisjuk, N., Borisjuk, L., Stadler, M., Stelzer, T., Schilde-Rentschler, L., Hemleben, V. Organization and distribution of different repeated genome elements in Solanum and their use as markers for the characterization of species and cultivars. Theor. Appl. Genet., 85: 801-808 (1993).
  20. Borisjuk, N., Hemleben, V. Nucleotide sequence of the potato rDNA intergenic spacer. Plant Mol. Biol., 21:381-384 (1993).
  21. Hemleben, V., Zentgraf, U., King, K., Borisjuk, N., Schweizer, G.. Middle repetitive and highly repetitive sequences detect polymorphisms in plants. In: Kahl G, Appelhans H, Kompf J, Driesel AJ. (Eds) DNA-Polymorphysms in Eukaryotic Genomes. Huthing, Heidelberg, p. 157-170 (1992).
  22. Kostenyuk, I., Lubaretz, O., Borisjuk, N., Voronin, V., Stoking, J., Gleba, Y. Isolation and characterization of intergeneric somatic hybrids in the
    Apocynaceae family. Theor. Appl. Genet., 82, 713-716 (1991).
  23. Volkov, R. A., Borisjuk, N. V., Kostyshin, S. S., Panchuk, I. I. Variability of rRNA genes during the chromosome constitution rearrangements in tobaccoes. Mol. Biology (Russian, English summary), 25, 442-449 (1991).
  24. Hemleben, V., Borisjuk, N., King, K., Schweizer, G., Torres, R. A., Ungried, K., Zentgraf, U. Middle repetitive and highly repetitive sequences detect polymorphisms in plants. Biotech Forum Europe, 8, 7/8, 437 (1991).
  25. Borisjuk, N. V., Miroshnichenko, G. P. Organization of ribosomal RNA genes in Brassica oleracea, B.campestris and their natural allotetraploid hybrid B. napus. Genetika (Russian, English summary), XXV, 3, 417-424, 1989.
  26. Borisjuk, N. V., Kostyshin, S. S., Volkov, R. A., Miroshnichenko, G. P. Ribosomal RNA gene organization in higher plants from Nicotiana genus. Mol. Biology (Russian, English summary), 1989, 23, 4, 1067-1075 (1989).
  27. Miroshnichenko, G. P., Borisjuk, N. V., Volkov, R. A. Organization of rDNA repeat units in the Solanaceae sexual and parasexual hybrids. Biochemistry (Russian, English summary), 54, 4, 669-675 (1989).
  28. Borisyuk, N. V., Momot, V. P., Gleba, Y. Novel class of rDNA repeat units in somatic hybrids between Nicotiana and Atropa. Theor. Appl. Genet., 76, 108-112 (1988).
  29. Borisjuk, N. V., Miroshnichenko, G. P. Structural organization of ribosomal DNA in Atropa belladonna. Biopolimers and Cell (USSR), 4, 101-105
    (1988).
  30. Borisyuk, N. V. Molecular-genetical constitution of somatic hybrid genomes (Review). In: “Plant Cell Engineering” (Ed. Y. Gleba). – Results of Science and Technology. Ser. Biotechnology, Moscow, v.9, p.73-113 (1988).
  31. Gleba, Y. Y., Hinnisdaels, S., Sidorov, V. A., Parokonny, A. S., Borisjuk, N. V., Cherep, Negrutiu, I., Jacobs, M. Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by “gamma-fusion”. Theor. Appl. Genet., 76, 760-766 (1988).
  32. Miroshnichenko, G. P., Borissyuk, N. V., Volkov, R. A., Gleba, Y. Y. Les sequences repetitives des AND chez Arabidopsis thaliana, Brassica campestris et leur hybride somatique, Arabidobrassica. Arabidopsis Inform Service (French, English summary), 26, 15-28 (1988).
  33. Borisjuk, N. V., Kanievsky, I. F., Gleba, Y. Y. Inheritance and organization of rDNA in somatic hybrids Nicotiana tabacum + Atropa belladonna. Proc. Acad. Sci. Ukr. SSR (Russian, English summary), Ser. B, 10, 60-63 (1987).
  34. Gleba, Y. Y., Kaleda, V. A., Parokonny, G. S., Borisjuk, N. V., Lopato, S. V., Negrutiu, I., Jacobs, I. Fertile intertribal nuclear hybrids Nicotiana + Atropa, obtaining by fusion of normal and irradiated somatic cells. Proc. Acad. Sci. USSR (Russian, English summary), 297,6, 1473-1475 (1987).
  35. Miroshnichenko, G. P., Volkov, R. A., Borisjuk, N. V. Genome structure of Brassica campestris, Arabidopsis thaliana and their somatic hybrid, Arabidobrassica. Biochemistry (Russian, English summary) 51, 1, 84-94 (1986).

 

  • US PATENT # 6,355,860 M. Borysyuk, L. Borysyuk, I. Raskin. Materials and methods for amplifying and enhanced transcribing of polynucleotides in plants and portions thereof. March 12, 2002.

  • US PATENT # 6,100,092 M. Borysyuk, L. Borysyuk, I. Raskin. Materials and methods for amplifying polynucleotides in plants. August 08, 2000.