Doloressa Gleba, Laboratory Researcher
Dr. Gleba’s 25 years of research experience in plant physiology, genetics and plant cell culture is reflected in more than 50 peer-review journal publications and presentations. She graduated from Vilnius State University, Lithuania, in 1971, with a M.Sc. in biology-chemistry and in 1979 received her Ph.D. in plant genetics from the Institute of Plant Physiology, USSR Academy of Sciences in Moscow. Her Ph.D. project was focused on the development of technology of suspension culture of Dioscorea deltoidea Wall. cells source of secondary metabolites – saponins.
After graduation, Dr. Gleba established a Plant Tissue Laboratory at the Institute of Botany, Academy of Sciences, Lithuania and worked as a Research Scientist developing methods of plant cell culture technologies such as rapid regeneration, mutant isolation and media optimization for plant breeding.
In 1979 she worked as a Research Scientist in the Laboratory of Plant Tissue Cultures at All-Union Institute of Sugar-Beet Breeding in Kiev, Ukraine, developing new varieties of sugar beets using new technologies – culture of isolated protoplasts, microspore culture as somatic hybridization.
During 1981-1984, as Senior Research Scientist at Botanical Garden of the Kiev State University, she was responsible for microclonal propagation and biotechnology of medicinal plants, and was a member of Scientific Council of Biological faculty and Botanical garden. Dr Gleba also participated in student teaching process as a supervisor for student diploma projects.
In 1990-1991 she was a post-doctoral trainee at Institute of Pharmaceutical Biology, Munich University in Munich, Germany were her research involved tissue cultures of species – producers of novel pharmaceuticals (Croton, Taxus).
As a Post-Doctoral Associate in Dr. Raskin’s Laboratory at Rutgers University, she was involved in various phytoremediation projects. In particular, she developed methods of plant screening for higher metal uptake as well as genetic improvement of plants for phytoremediation using somatic plant hybridization. Dr. Gleba was involved in all aspects of research, starting with experimental design, carrying out experiments, data analysis and recommending future research; she presented results at regular laboratory meetings, Biotech seminars and at Scientific Advisors Meetings at Phytotech, Inc.
Based on her extensive knowledge in plant physiology, microbiology and plant cell culture, Doloressa successfully developed methods of screening plant leave cuticle washings, plant root extracts and exudates for antimicrobial and antifungal activity for new drug discovery.
Dr. Gleba’s main research interests are in the scientific field of plant cell biology and genetics; plant physiology; phytoremediation; plant microclonal propagation and plant biotechnologies for conservation of biodiversity; natural products.
Recent Publications
Gleba D., Momot V., Okolot A., Gleba Y., 1985. Effect of para-flurophenyl alanine on nuclear DNA content and
chromosome segregation in hybrid cells of Nicotiana+ Atropa. Cytology and Genetics, 19, pp. 281-285.
Rudzianskaite A., Gleba D., Cieminis K. 1985. Incorporation 3H-thymidine in tobacco protoplasts. Proc. Acad. Sci. Lithuanian SSR, 17, p.1102-1104.
Gleba D. 1987. Use in vitro tissue culture methods in plant introduction. Abstr. 8 Ukrainian Botanical Society, Kiev, p. 209.
Gleba D., Gleba Y. 1987. Hybridization of somatic plant cells. Abstr. 5 All-Union Soc. of geneticists and breeders. Vol. VI, p. 131.
Gleba D.,Gordzievskaya L. 1987. Propagation of Platycerium bifurcatum(Cav) in in vitro culture. Abstr. Conf. "Introduction and acclimatization of plants", Donetsk, Ukraine, vol.7, p. 59-61.
Gleba D., Kaleda L., Parakony A., Borysyuk N., Gleba Y., Negrutiu I. 1987. Nuclear genetics of somatic cell hybrids. Abstr. 14 Intern. Bot. Congr., West Berlin.
Gleba D., Stupnitsky V. 1988. Use of in vitro culture methods for propagation of subtropical and green-house plants. Abstr. Int.Conf.
"Biology of cultured cells and biotechnology", Novosibirsk, Rusia, p. 323-324.
Stupnitsky V., Gleba D. 1988. Introduction of in vitro morphogenesis in Musa nana. Abstr. Int.Conf. "Biology of cultured cells and biotechnology", Novosibirsk, Rusia,p. 325-326.
Gleba D., Stupnitsky V. 1989. Propagation of Cuningammia in in vitro culture. Abstr. Conf. "Role of Botanical gardens in preservation and enrichment of world flora", Kiev, vol.2, p.138.
Stupnitsky V., Gleba D. 1989. Generative multiplication of Bletilla striota Reinchen. in sterile culture. Abstr. Conf. "Role of Botanical gardens in preservation and enrichment of world flora", Kiev, vol.2, p.148.
Gleba D., Stupnitsky V. 1989. Development of technology of microclonal propagation of higher plants in Hortus Botanicus Fominianus. In: "Conservation, study and enrichment of world flora", Kiev, Ukraine, vol.16, p. 66-70.
Gleba D., Stupnitsky V. 1990. Use of in vitro methods for propagation of subtropical green-house plants. Abstr. VII Intern. Congr. Plant Tissue Cell Culture. Amsterdam, A-3-74, p.101.
Gleba D., Stupnitsky V., Gordzievskaya L. 1991.Propagation of several fern species in vitro. In: "Conservation, study and enrichment of world flora", Kiev, Ukraine, vol.18, p. 112-117.
Doloressa Gleba, David E. Salt, Robert Smith, Yoram Kapulnik, Burt Ensley and Ilya Raskin. 1996. Screening for metal accumulating plants. Abstr. Ann. Meet .of AAPS, San Antonio, Texas, p. 552.
Ilya Raskin, Doloressa Gleba, Robert Smith, David E. Salt, Slavik Dushenkov, Yoram Kapulnik and Burt Ensley. 1996. Using plant seedlings to remove heavy metals from water. Abstr. Ann. Meet .of AAPS, San Antonio, Texas, p. 552.
David E. Salt, Ingrid J. Pickering, Roger C. Prince, Doloressa Gleba, Slavik Dushenkov, Robert D. Smith and Ilya Raskin. 1997. Metal accumulation by aquacultured seedlings of Indian mustard. Environ. Sci. Tech., 31, p.1636-1644.
Slavik Dushenkov, Dev Vasudev, Yoram Kapulnik, Doloressa Gleba, David Fleishner, K.C. Ting and Burt Ensley.1997. Removal of Uranium from water using terrestrial plants. Environ. Sci. Tech., 31, p.3468-3474.
Gleba D., Gleba, Y., M. Skarzhinskaya, K. Glimelius and I. Raskin. 1998. Transfer of resistance to heavy metals from Thlaspi to Brassica by asymmetric hybridization. Abstr. IX Int. Congr. On Plant Tissue Culture, Jerusalem, p. 145.
Doloressa Gleba, Nicolai V. Borisjuk, Liudmila G. Borisjuk, Ralf Kneer, Alexander Poulev, Marina Skarzhinskaya, Slavik Dushenkov, Sithes Logendra, Yuri Y. Gleba and Ilya Raskin.1999. Use of plant roots for phytoremediation and molecular farming. Proc. Natl Acad. Sci. USA Vol. 96, pp. 5973-5977.