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Ljudmila Borysyuk, Postdoctoral Associate

Ljudmila BorysyukDr. Ljudmila Borysyuk has over 15 years experience of academic and applied research in microbiology, plant molecular physiology and biotechnology. During 1980-1990 Dr. Borysyuk studied physiology of melanin containing fungi under different extreme conditions including the exposure to high radiation after the Chernobyl nuclear plant accident.

Since 1991, she shifted her focus to plant molecular biology and physiology, acquiring solid laboratory experience at the University of Tübingen. In 1993, Dr. Borysyuk joined the Institute of Plant Genetics, (Gatersleben), where she investigated tissue specific promoter activiites during embryogenesis by in situ hybridization of mRNA and immunodetection of proteins. She designed a pioneer method of high-resolution histographical mapping of glucose concentration in plant tissues.

In 1996, Dr. Borysyuk moved to the Biotech Center of Rutgers, working as a research scientist for Phytomedics Inc. She established an original plant cultivation system, designed methods for the deection of protein activities (xylanase, SEAP, GFP) secreted into the hydroponic medium. She significantly contributed to the development of a novel plant transformation system, and thoroughly tested its activity at DNA, mRNA and protein expression levels.

PubMed Bibliography

Publications

  1. Martin Mascher, Heidrun Gundlach, Axel Himmelbach, Sebastian Beier, Sven O. Twardziok, Thomas Wicker, Volodymyr Radchuk, Christoph Dockter, Pete E. Hedley, Joanne Russell, Micha Bayer, Luke Ramsay, Hui Liu, Georg Haberer, Xiao-Qi Zhang, Qisen Zhang, Roberto A. Barrero, Lin Li, Stefan Taudien, Marco Groth, Marius Felder, Alex Hastie, Hana Šimková, Helena Staňková, Jan Vrána, Saki Chan, María Muñoz-Amatriaín, Rachid Ounit, Steve Wanamaker, Daniel Bolser, Christian Colmsee, Thomas Schmutzer, Lala Aliyeva-Schnorr, Stefano Grasso, Jaakko Tanskanen, Anna Chailyan, Dharanya Sampath, Darren Heavens, Leah Clissold, Sujie Cao, Brett Chapman, Fei Dai, Yong Han, Hua Li, Xuan Li, Chongyun Lin, John K. McCooke, Cong Tan, Penghao Wang, Songbo Wang, Shuya Yin, Gaofeng Zhou, Jesse A. Poland, Matthew I. Bellgard, Ljudmilla Borisjuk, Andreas Houben, Jaroslav Doležel, Sarah Ayling, Stefano Lonardi, Paul Kersey, Peter Langridge, Gary J. Muehlbauer, Matthew D. Clark, Mario Caccamo, Alan H. Schulman, Klaus F. X. Mayer, Matthias Platzer, Timothy J. Close, Uwe Scholz, Mats Hansson, Guoping Zhang, Ilka Braumann, Manuel Spannagl, Chengdao Li, Robbie Waugh, Nils Stein. A chromosome conformation capture ordered sequence of the barley genome. Nature, Vol. 544, pp. 427-433, 2017.
  2. Woodfield, H. K., Sturtevant, D., Borisjuk, L., Munz, E., Guschina, I. A., Chapman, K., Harwood, J. L. Spatial and temporal mapping of key lipid species in Brassica napus seeds. Plant Physiology, Vol. 173, No. 4, pp. 1998-2009, 2017.
  3. Munz, E., Jakob, P. M., Borisjuk, L.. The potential of nuclear magnetic resonance to track lipids in planta. Biochimie, Vol. 130, pp. 97-108, 2016.
  4. Kovalchuk, N., Chew, W., Sornaraj, P., Borisjuk, N., Yang, N., Singh, R., Bazanova, N., Shavrukov, Y., Gündel, A., Munz, E., Borisjuk, L., Langridge, P., Hrmova, M., Lopato, S. The homeodomain transcription factor TaHDZipI-2 from wheat regulates frost tolerance, flowering time and spike development in transgenic barley. New Phytologist, Vol. 211, No. 2, pp. 671-687, 2016.
  5. Rolletschek, H., Grafahrend-Belau, E., Munz, E., Radchuk, V., Kratäusch, R., Tschiersch, H., Melkus, G., Jakob, P. M., Borisjuk, L. Metabolic architecture of the cereal grain and its relevance to maximize carbon use efficiency. Plant Physiology, Vol. 169, No. 3, pp. 1698-1713, 2015.
  6. Schwender, J., Hebbelmann, I., Heinzel, N., Hildebrandt, T., Rogers, A., Naik, D., Klapperstück, M., Braun, H.-P., Schreiber, F., Denolf, P., Borisjuk, L., Rolletschek, H. Quantitative multilevel analysis of central metabolism in developing oilseeds of oilseed rape during in vitro culture. Plant Phyiology, Vol. 168, No. 3, pp. 828-848, 2015.
  7. Fuchs, J., Melkus, G., Borisjuk, L., Jakob, P. Tracking metabolite dynamics in plants via indirect 13C chemical shift imaging with an interleaved variable density acquisition weighted sampling pattern. Magnetic Resonance Materials in Physics, Biology and Medicine, Vol. 28, No. 2, pp. 127-134, 2015.
  8. Rolletschek, H., Fuchs, J., Friedel, S., Börner, A., Todt, H., Jakob, P. M., Borisjuk, L. A novel noninvasive procedure for high-throughput screening of major seed traits. Plant Biotechnology Journal, Vol. 13, No. 2, pp. 188-199, 2015.
  9. Schwender, J., König, C., Klapperstück, M., Heinzel, N., Munz, E., Hebbelmann, I., Hay, J. O., Denolf, P., De Bodt, S., Redestig, H., Caestecker, E., Jakob, P. M., Borisjuk, L., Rolletschek, H. Transcript abundance on its own cannot be used to infer fluxes in central metabolism. Frontiers in Plant Science, Vol. 5, article 668, 2014.
  10. Radchuk, V., Borisjuk, L. Physical, metabolic and developmental functions of the seed coat. Frontiers in Plant Science, Vol. 5, article 510, 2014.
  11. Borisjuk, L., Rolletschek, H., Neuberger, T. Nuclear magnetic resonance imaging of lipid in living plants. Progress in Lipid Research, Vol. 52, No. 4, pp. 465-487, 2013.
  12. Horn, P. J., Silva, J. E., Anderson, D., Fuchs, J., Borisjuk, L., Nazarenus, T., Shulaev, V., Cahoon, E. B., Chapman, K. D. Imaging heterogeneity of membrane and storage lipids in transgenic Camelina sativa seeds with altered fatty acid profiles. The Plant Journal, Vol. 76, No. 1, pp. 138-150, 2013.
  13. Verboven, H., Herremans, E., Borisjuk, L., Helfen, L., Quang Tri Ho, Tschiersch, H., Fuchs, J., Nicolaï, B. M., Rolletschek, H. Void space inside the developing seed of Brassica napus and the modelling of its function New Phytologist, Vol. 199, No. 4, pp. 936-947, 2013.
  14. Borisjuk, L., Neuberger, T., Schwender, J., Heinzel, N., Sunderhaus, S., Fuchs, J., Hay, J. O., Tschiersch, H., Braun, H.-P., Denolf, P., Lambert, B., Jakob, P. M., Rolletschek, H. Seed Architecture Shapes Embryo Metabolism in Oilseed Rape. The Plant Cell, Vol. 25, No. 5, pp. 1625-1640, 2013.
  15. Fuchs, J., Neuberger, T., Rolletschek, H., Schiebold, S., Thuy Ha Nguyen, Borisjuk, N., Börner, A., Melkus, G., Jakob, P, Borisjuk, L. A noninvasive platform for imaging and quantifying oil storage in submillimeter Tobacco seed. Plant Physiology, Vol. 161, No. 2, pp. 583-593, 2013.
  16. Tschiersch, H., Liebsch, G., Borisjuk, L., Stangelmayer, A. An imaging method for oxygen distribution, respiration and photosynthesis at a microscopic level of resolution. New Phytologist, Vol. 196, No. 3, pp. 926-936, 2012.
  17. Borisjuk, L., Rolletschek, H., Neuberger, T. Surveying the plant’s world by magnetic resonance imaging. The Plant Journal, Vol. 70, No. 1, pp. 129-146, 2012.
  18. Radchuk, V., Kumlehn, J., Rutten, T., Sreenivasulu, N., Radchuk, R., Rolletschek, H., Herrfurth, C., Feussner, I., Borisjuk, L. Fertility in barley flowers depends on Jekyll functions in male and female sporophytes. New Phytologist, Vol. 194, No. 1, pp. 142-157, 2012.
  19. Horn, P., Korte, A. R., Neogi, P. B., Love, E., Fuchs, J., Strupat, K., Borisjuk, L., Shulaev, V., Lee, Y.-J., Chapman, K. D. Spatial mapping of lipids at cellular resolution in embryos of cotton. The Plant Cell, Vol. 24, No. 2, pp. 622-636, 2012.
  20. Melkus, G., Rolletschek, H., Fuchs, J., Radchuk, V., Grafahrend-Belau, E., Sreenivasulu, N., Rutten, T., Weier, D., Heinzel, N., Schreiber, F., Altmann, T., Jakob, P. M., Borisjuk, L. Dynamic 13C/1H NMR imaging uncovers sugar allocation in the living seed. Plant Biotechnology Journal, Vol. 9, No. 9, pp. 1022-1037, 2011.
  21. Jasik, J., Schiebold, S., Rolletschek, H., Denolf, P., Van Adenhove, K., Altmann, T., Borisjuk, L. Subtissue-specific evaluation of promoter efficiency by quantitative fluorometric assay in laser microdissected tissues of rapeseed. Plant Physiology, Vol. 157, No. 2, pp. 563-573, 2011.
  22. Hayden, D. M., Rolletschek, H., Borisjuk, L., Corwin, J., Kliebenstein, D. J., Grimberg, A., Stymne, S., Dehesh, K. Cofactome analyses reveal enhanced flux of carbon into oil for potential biofuel production. The Plant Journal, Vol. 67, No. 6, pp. 1018-1028, 2011.
  23. Borisjuk, L., Rolletschek, H., Fuchs, J., Melkus, G., Neuberger, T. Low and high field magnetic resonance for in vivo analysis of seeds. Materials, Vol. 4, No. 8, pp. 1426-1439, 2011.
  24. Rolletschek, H., Melkus, G., Grafahrend-Belau, E., Fuchs, J., Heinzel, N., Schreiber, F., Jakob, P. M., Borisjuk, L. Combined noninvasive imaging and modeling approaches reveal metabolic compartmentation in the barley endosperm. The Plant Cell, Vol. 23, No. 8, pp. 3041-3054, 2011.
  25. Schiebold, S., Tschiersch, H., Borisjuk, L., Heinzel, N., Radchuk, R., Rolletschek, H. A novel procedure for the quantitative analysis of metabolites, storage products and transcripts of laser microdissected seed tissues of Brassica napus. Plant Methods, Vol. 7, No. 1, pp. 1-13, 2011.
  26. Thiel, J., Rolletschek, H., Friedel, S., Lunn, J. E., Thuy Ha Nguyen, Feil, R., Tschiersch, H., Müller, M., Borisjuk, L. Seed-specific elevation of non-symbiotic hemoglobin AtHb1: beneficial effects and underlying molecular networks in Arabidopsis thaliana. BMC Plant Biology, Vol. 11, No. 1, pp. 48-66, 2011.
  27. Tschiersch, H., Borisjuk, L., Rutten, T., Rolletschek, H. Gradients of seed photosynthesis and its role for oxygen balancing. Biosystems, Vol. 103, No. 2, pp. 302-308, 2011.
  28. Sreenivasulu, N., Radchuk, V., Alawady, A., Borisjuk, L., Weier, D., Staroske, N., Fuchs, J., Miersch, O., Strickert, M., Usadel, B., Wobus, U., Grimm, B., Weber, H., Weschke, W. De-regulation of abscisic acid contents causes abnormal endosperm development in the barley mutant seg8. The Plant Journal, Vol. 64, No. 4, pp. 589-603, 2010.
  29. Sreenivasulu, N., Borisjuk, L., Junker, B. H., Mock, H.-P., Rolletschek, H., Seiffert, U., Weschke, W., Wobus, U. Chapter 2 – Barley Grain Development: Toward an Integrative View. In: International Review of Cell and Molecular Biology, ISBN: 978-0-12-381258-2, Kwang W. Jeon Editor, Vol. 281, pp. 49-89, 2010.
  30. Melkus, G., Rolletschek, H., Radchuk, R., Fuchs, J., Rutten, T., Wobus, U., Altmann, T., Jakob, P. M., Borisjuk, L. The metabolic role of the legume endosperm: a noninvasive imaging study. Plant Physiology, Vol. 151, No. 3, pp. 1139-1154, 2009.
  31. Borisjuk, N., Borisjuk, L., Komarnytsky, S., Timeva, S., Hemleben, V., Gleba, Y., Raskin, I. Tobacco ribosomal DNA spacer element stimulates amplification and expression of heterologous genes. Nature Biotechnology, Vol. 18, pp. 1303-1306, 2000.
  32. Borisjuk, N., Borisjuk, L., Logendra, S., Petersen, F., Gleba, Y., Raskin, I. Production of recombinant proteins in plant root exudates. Nature Biotechnology, Vol. 17, pp. 466-469, 1999.
  33. Gleba, D., Borisjuk, N., Borisjuk, L., Kneer, R., Poulev, A., Skarzhinskaya, M., Dushenkov, S., Logendra, S., Gleba, Y., and Raskin, I. Use of plant roots for phytoremediation and molecular farming. Proceedings of the National Academy of Sciences, Vol. 96, No. 11, pp. 5973-5977, 1999.
  34. Borisjuk, L., Walenta, S., Weber, H., Müller-Klieser, W., and Wobus, U. High-resolution histographical mapping of glucose concentrations in developing cotyledons of Vicia faba in relation to mitotic activity and storage processes: glucose as a possible developmental trigger. Plant J. (cover story), Vol. 15, No. 4, pp. 583-591, 1998.
  35. Weber, H., Heim, U., Golombek, S., Borisjuk, L., Manteuffel, R., Wobus, U. Expression of a yeast-derived invertase in developing cotyledons of Vicia narbonensis alters the carbohydrate state and affects storage functions. Plant J., Vol. 16, No. 2, pp. 163-172, 1998.
  36. Weber, H., Heim, U., Golombek, S., Borisjuk, L., Wobus, U. Assimilate uptake and the regulation of seed development. Seed Science Research, Vol. 8, No. 3, pp. 331-346, 1998.
  37. Borisjuk, N., Sitailo, L., Borisjuk, L., Adler, K., Malysheva, L., Tewes, A., and Manteuffel, R. Calreticulin in plant cells: primary structure, developmentally regulated expression and intracellular distribution. Planta 206: 504-514, 1998.
  38. Weber, H., Golombek, S., Heim, U., Borisjuk L., Panitz, R., Manteuffel, R., Wobus, U. Integration of carbohydrate and nitrogen metabolism during legume development – implication for storage product synthesis. J. Plant Physiol 152(6):641-648, 1998.
  39. Weber, H.,Borisjuk, L., Heim, U., Sauer, N., Wobus, U. A role of sugar transporters during seed development: molecular characterization of a hexose and a sucrose carrier in faba bean seeds. Plant Cell 9, 895-908, 1997.
  40. Weber, H., Borisjuk, L., Wobus, U. Sugar import and metabolism during seed development (review). Trends in Plant Science, 2, 169-174, 1997.
  41. Wobus, U., Borisjuk, L., Heim, U., Weber, H. Ein Kapitel Metamorphose der Pflanzen; Embryogenese und Samenentwicklung. Leopoldina (R.3) 42: 297-304, 1997.
  42. Balzer, H. J., Borisiuk, L., Meyer, H. M., Matzk, F., Baumlein, H. A pollen allergen-encoding gene is expressed in wheat ovaries. Plant Molecular Biology. 32 (3) p. 435-445, 1996.
  43. Weber, H., Buchner, P., Borisjuk L., Wobus, U. Sucrose methabolism during cotyledon development of Vicia faba L. is controlled by the concerted action of both sucrose – phosphate synthase and sucrose synthase: expression pattern, metabolic regulation and implications for seed development. Plant J. 9, 841-850, 1996.
  44. Buchner, P., Borisjuk, L., Wobus, U. Glucan phosphorylases in Vicia faba L.: cloning, structural analysis and expression patterns of cytosolic and plastidic forms in relation to starch. Planta, 199, 64-73, 1996.
  45. Borisjuk, L., Weber, H., Heim, U., Wobus, U. Transfer cell-specific expression of a putative sucrose – H+ symporter in developing embryos of Vicia faba. Plant Physiol. Biochem. (Special issue) 180, 1996.
  46. Wobus, U., Borisjuk, L., Panitz, R., Manteuffel, R., Bäumlein, H., Wohlfarth, T., Heim, U., Weber, H., Misera, S., Weschke, W. Control of seed storage protein gene expression: New aspects on an old problem. J. Plant Physiol. 145, 592-599, 1995.
  47. Weber, H., Borisjuk, L., Heim, U., Wobus, U. Seed coat associated invertases of Vicia faba control both unloding and storage functions: cloning of cDNAs and cell-type specific expression. Plant Cell, 7, 1835-1846, 1995.
  48. Weber, H., Borisjuk, L., Wobus, U. Cell-type specific, coordinate expression of two ADP-glucose pyrophosphorylase genes in relation to starch biosynthesis during seed development of Vicia faba L. Planta, 105, 352-361, 1995.
  49. Panitz, R., Borisjuk, L., Manteuffel, R., Wobus, U. Transient expression of storage-protein genes during early embryogenesis of Vicia faba: synthesis and metabolization of vicilin and legumin in the embryo, suspensor and endosperm. Planta 197, 765-774, 1995.
  50. Borisjuk, L., Weber, H., Panitz, R., Manteuffel, R., Wobus, U. Embryogenesis of Vicia faba L.: histo-differentiation in relation to starch and storage protein synthesis. J. Plant Physiol. 147, 203-218, 1995.
  51. Borisjuk, N., Borisjuk, L., G. Petjuch and V. Hemleben. Comparison of nuclear ribosomal RNA genes among Solanum species and other Solanaceae. Genome 37, 271-279, 1994.
  52. Schweizer, G., Borisjuk, N., Borisjuk, L., Stadler, M., Stelzer, T., Schilde-Rentschler, L., Hemleben, V. Organization and distribution of different repeated genome elements in Solanum and their use as markers for the characterization of species and cultivars. Theor. Appl. Genet., 85: 801-808, 1993.
  53. Borisjuk, L., Arzatbanov, V. Yu., Zdanova, N. N. Effect of electron chain inhibitors on the respiration and growth of Cladosporium cladosporioides and its albino mutant. Izvestija Acad. Sci. USSR., 3, 1992.
  54. Borisjuk, L., Kharatjan, E. F., Zdanova, N. N. Localization and dinamics of melanine accumulation in cells of Cladosporium cladosporioides (Fresen) de Vries. Microbiol. J. (Russian), 53, 6, 10-16, 1991.
  55. Zdanova, N. N., Borisjuk, L., Arzatbanov, V. Yu. Occurence of the K-Type of Life Strategy in some melanin-containing fungi under experimental conditions. Folia Microbiol., 35, 423-430, 1990.
  56. Zdanova, N. N., Rozshko, I. I., Borisjuk, L. Assimilation of radioactive carbon from 14CO2 by Cladosporium cladosporioides in dark and on the light. Mycol. Phytopatol., 19, 2, 132-139, 1985.