{"id":757,"date":"2019-11-03T23:45:22","date_gmt":"2019-11-03T23:45:22","guid":{"rendered":"http:\/\/sites.rutgers.edu\/ilya-raskin-lab\/?page_id=757"},"modified":"2019-11-04T00:22:22","modified_gmt":"2019-11-04T00:22:22","slug":"nikolai-borysyuk","status":"publish","type":"page","link":"https:\/\/sites.rutgers.edu\/ilya-raskin-lab\/nikolai-borysyuk\/","title":{"rendered":"Nikolai Borysyuk, Assistant Research Professor"},"content":{"rendered":"

I have over 15 years experience of academic and applied innovative research in the area of molecular biology and biotechnology. During 1982-1989, after graduating from Kiev State University, I worked on the inheritance and molecular organization of rRNA genes in somatic hybrids getting a solid practice in plant molecular biology at the Moscow State University. In 1990 I was awarded with the prestigious A. von Humboldt Fellowship, which allowed me to continue my academic study on the evolution of ribosomal RNA genes and to initiate a practically-oriented project on the construction of plant high-copy-number transformation vectors at the University of Tübingen, Germany.<\/p>\n

I joined the Institute of Plant Genetics, (Gatersleben) in 1992, where I organized a molecular biology group for studying gene expression during plant development, initiated and managed two international projects. In particular, I developed an effective method for the isolation of embryogenesis-related genes, cloned and characterized a number of genes including a plant chaperon, calreticulin, important for correct protein folding and secretion.<\/p>\n

In 1996, I joined the Biotech Center of Rutgers University, working as a Phytomedics scientist in Dr. Raskin’s laboratory. Supervising a research group, I contributed to the development of new technologies for the production of recombinant proteins, based on the rhizosecretion from hydroponically grown plants. Rhizosecretion which offers a low-cost, large-scale recombinant protein production system became the basic technology of Phytomedics. In addition, I invented a method for the construction of a new plant transformation vector to significantly increase expression of valuable pharmaceutical proteins in transgenic plants.<\/p>\n

Publications<\/strong><\/h2>\n
    \n
  1. Komarnytsky, S., N. I. Borisjuk<\/strong>, N. Yakoby, A. Garvey and I. Raskin. Co-secretion of proteinase inhibitor enhances stability of antibodies produced by plant roots. Plant Physiol., 141: pp. 1185-1193<\/a>, 2006.<\/li>\n
  2. Komarnytsky, S., Gaume, A., Garvey, A., Borisjuk, N.<\/strong>, Raskin, I. Quick and efficient system for antibiotic-free expression of heterologous genes in tobacco roots. Plant Cell Reports, 22: 765-773, 2004.<\/li>\n
  3. Komarnytsky, S., Borisjuk, N<\/strong>. Functional analysis of promoter elements in plants. In: Genetic Engineering: Principles and Methods (Ed. J. K. Setlow), vol. 23, pp. 113-141, (2003).<\/li>\n
  4. Volkov, R., Panchuk, I., Borisjuk, L., Borisjuk, N.<\/strong> Ribosomal DNA in plants: molecular organization, evolution and applications (Review). Cytology and Genetics, (Ukrainian, English summary), 1: 72-79, 2003.<\/li>\n
  5. Gaume, A., Komarnytsky, S., Borisjuk, N.<\/strong>, Raskin, I. Rhizosecretion of recombinant proteins from plant hairy roots. Plant Cell Reports, 21: 1188-1193, 2003.<\/li>\n
  6. Ilya Raskin, David M. Ribnicky, Slavko Komarnytsky, Nebojša Ilić, Alexander Poulev, Nikolai Borisjuk<\/strong>, Anita Brinker, Diego A. Moreno, Christophe Ripoll, Nir Yakoby, Joseph M. O’Neal, Teresa Cornwell, Ira Pastor and Bertold Fridlender. Plants and human health in the twenty-first century. Trends in Biotechnology, 2002, 20:12<\/i>:522-531.<\/li>\n
  7. Borisjuk, N.<\/strong>, Borisjuk, L., Komarnytsky, S., Timeva, S., Hemleben, V., Gleba, Y., Raskin, I. Tobacco ribosomal DNA spacer element stimulates amplification and expression of heterologous genes. Nature Biotechnology, 18: 1303-1306 (2000).<\/li>\n
  8. Komarnytsky, S., Borisjuk, N.<\/strong>, Borisjuk, L., Alam, M., Raskin, I. Production of recombinant proteins in the guttation fluid. Plant Physiology, 124: 1-7 (2000).<\/li>\n
  9. Borisjuk, N.<\/strong>, Borisjuk, L., Logendra, S., Petersen, F., Gleba, Y., Raskin, I. Production of recombinant proteins in plant root exudates. Nature Biotechnology, 17: 466-469 (1999).<\/li>\n
  10. Gleba, D., Borisjuk, N.<\/strong>, Borisjuk, L., Kneer, R., Poulev, A., Skarzhinskaya, M., Dushenkov, S., Logendra, S., Gleba, Y., Raskin, I. Radical approaches to phytoremediation and molecular farming. Proc. Nat. Acad. Sci. USA, 96: 5973-5977 (1999).<\/li>\n
  11. Volkov, R., N. Borisjuk<\/strong>, I. Panchuk, D. Schweizer, and V. Hemleben. Elimination and rearrangement of parental rDNA in the allotetraploid Nicotiana
    \ntabacum<\/i>. Mol. Biol. Evol. 16: 311-320 (1999).<\/li>\n
  12. Borisjuk, N.<\/strong>, L. Sitailo, A. Tewes and R. Manteuffel. Defferential expression and sequence analysis of three ribosomal protein S17, L15 and S5 induced during the early stages of somatic embryogenesis in Nicotiana plumbaginifolia<\/i>. Prepared for publication in Molec Gen Genet (1999).<\/li>\n
  13. Borisjuk, N.<\/strong>, L. Sitailo, L. Borisjuk, K. Adler, L. Malysheva, A. Tewes and R. Manteuffel. Calreticulin in plant cells: primary structure, developmentally regulated expression and intracellular distribution. Planta 206: 504-514 (1998).<\/li>\n
  14. Borisjuk, N.<\/strong>, Davidjuk, I., Kostyshin, S., Miroshnichenko, G., Velasko, R., Hemleben, V., Volkov, R. Structural analysis of rDNA in the genus Nicotiana<\/i>. Plant Mol Biol 35, 655-660 (1997).<\/li>\n
  15. Friesen, N., Borisjuk, N.<\/strong>, Mes, T., Klaas, M., Hanelt, P. Allotetraploid origin of Allium altyncolicum<\/i> (Alliaceae, Allium <\/i>sect. Schoenoprasum<\/i>) as investigated by kariological and molecular markers. Plant Systematics and Evolution 206, 317-335 (1997).<\/li>\n
  16. Zanke, C., Borisjuk, N.<\/strong>, Ruoss, B., Schilde-Rentschler, L., Ninnemann, H., Hemleben, V. A specific oligonucleotide of the 5S rDNA spacer and species-specific elements identify symmetric somatic hybrids between Solanum tuberosum<\/i> and S. pinnatisectum<\/i>. Theor. Appl. Genet. 90, 720-726 (1995).<\/li>\n
  17. Stadler, M., Stelzer, T., Borisjuk, N.<\/strong>, Zanke, C., Schilde-Rentschler, L., Hemleben, V. Distribution of novel and known repeated elements of Solanum<\/i> and application for the identification of somatic hybrids among Solanum<\/i> species. Theor. Appl. Genet., 91, 1271-1278 (1995).<\/li>\n
  18. Borisjuk, N.<\/strong>, Borisjuk, L., Petjuch, G., Hemleben, V. Comparison of nuclear ribosomal RNA genes among Solanum<\/i> species and other Solanaceae<\/i>. Genome 37, 271-279 (1994).<\/li>\n
  19. Schweizer, G., Borisjuk, N.<\/strong>, Borisjuk, L., Stadler, M., Stelzer, T., Schilde-Rentschler, L., Hemleben, V. Organization and distribution of different repeated genome elements in Solanum<\/i> and their use as markers for the characterization of species and cultivars. Theor. Appl. Genet., 85: 801-808 (1993).<\/li>\n
  20. Borisjuk, N.<\/strong>, Hemleben, V. Nucleotide sequence of the potato rDNA intergenic spacer. Plant Mol. Biol., 21:381-384 (1993).<\/li>\n
  21. Hemleben, V., Zentgraf, U., King, K., Borisjuk, N.<\/strong>, Schweizer, G.. Middle repetitive and highly repetitive sequences detect polymorphisms in plants. In: Kahl G, Appelhans H, Kompf J, Driesel AJ. (Eds) DNA-Polymorphysms in Eukaryotic Genomes. Huthing, Heidelberg, p. 157-170 (1992).<\/li>\n
  22. Kostenyuk, I., Lubaretz, O., Borisjuk, N.<\/strong>, Voronin, V., Stoking, J., Gleba, Y. Isolation and characterization of intergeneric somatic hybrids in the
    \nApocynaceae<\/i> family. Theor. Appl. Genet., 82, 713-716 (1991).<\/li>\n
  23. Volkov, R. A., Borisjuk, N. V.<\/strong>, Kostyshin, S. S., Panchuk, I. I. Variability of rRNA genes during the chromosome constitution rearrangements in tobaccoes. Mol. Biology (Russian, English summary), 25, 442-449 (1991).<\/li>\n
  24. Hemleben, V., Borisjuk, N.<\/strong>, King, K., Schweizer, G., Torres, R. A., Ungried, K., Zentgraf, U. Middle repetitive and highly repetitive sequences detect polymorphisms in plants. Biotech Forum Europe, 8, 7\/8, 437 (1991).<\/li>\n
  25. Borisjuk, N. V.<\/strong>, Miroshnichenko, G. P. Organization of ribosomal RNA genes in Brassica oleracea<\/i>, B.campestris<\/i> and their natural allotetraploid hybrid B. napus<\/i>. Genetika (Russian, English summary), XXV, 3, 417-424, 1989.<\/li>\n
  26. Borisjuk, N. V.<\/strong>, Kostyshin, S. S., Volkov, R. A., Miroshnichenko, G. P. Ribosomal RNA gene organization in higher plants from Nicotiana<\/i> genus. Mol. Biology (Russian, English summary), 1989, 23, 4, 1067-1075 (1989).<\/li>\n
  27. Miroshnichenko, G. P., Borisjuk, N. V.<\/strong>, Volkov, R. A. Organization of rDNA repeat units in the Solanaceae sexual and parasexual hybrids. Biochemistry (Russian, English summary), 54, 4, 669-675 (1989).<\/li>\n
  28. Borisyuk, N. V.<\/strong>, Momot, V. P., Gleba, Y. Novel class of rDNA repeat units in somatic hybrids between Nicotiana<\/i> and Atropa<\/i>. Theor. Appl. Genet., 76, 108-112 (1988).<\/li>\n
  29. Borisjuk, N. V.<\/strong>, Miroshnichenko, G. P. Structural organization of ribosomal DNA in Atropa belladonna<\/i>. Biopolimers and Cell (USSR), 4, 101-105
    \n(1988).<\/li>\n
  30. Borisyuk, N. V<\/strong>. Molecular-genetical constitution of somatic hybrid genomes (Review). In: “Plant Cell Engineering” (Ed. Y. Gleba). – Results of Science and Technology. Ser. Biotechnology, Moscow, v.9, p.73-113 (1988).<\/li>\n
  31. Gleba, Y. Y., Hinnisdaels, S., Sidorov, V. A., Parokonny, A. S., Borisjuk, N. V.<\/strong>, Cherep, Negrutiu, I., Jacobs, M. Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia<\/i> and Atropa belladonna<\/i> obtained by “gamma-fusion”. Theor. Appl. Genet., 76, 760-766 (1988).<\/li>\n
  32. Miroshnichenko, G. P., Borissyuk, N. V.<\/strong>, Volkov, R. A., Gleba, Y. Y. Les sequences repetitives des AND chez Arabidopsis thaliana<\/i>, Brassica campestris<\/i> et leur hybride somatique, Arabidobrassica<\/i>. Arabidopsis Inform Service (French, English summary), 26, 15-28 (1988).<\/li>\n
  33. Borisjuk, N. V.<\/strong>, Kanievsky, I. F., Gleba, Y. Y. Inheritance and organization of rDNA in somatic hybrids Nicotiana tabacum<\/i> + Atropa belladonna<\/i>. Proc. Acad. Sci. Ukr. SSR (Russian, English summary), Ser. B, 10, 60-63 (1987).<\/li>\n
  34. Gleba, Y. Y., Kaleda, V. A., Parokonny, G. S., Borisjuk, N. V.<\/strong>, Lopato, S. V., Negrutiu, I., Jacobs, I. Fertile intertribal nuclear hybrids Nicotiana + Atropa<\/i>, obtaining by fusion of normal and irradiated somatic cells. Proc. Acad. Sci. USSR (Russian, English summary), 297,6, 1473-1475 (1987).<\/li>\n
  35. Miroshnichenko, G. P., Volkov, R. A., Borisjuk, N. V<\/strong>. Genome structure of Brassica campestris<\/i>, Arabidopsis thaliana<\/i> and their somatic hybrid, Arabidobrassica<\/i>. Biochemistry (Russian, English summary) 51, 1, 84-94 (1986).<\/li>\n<\/ol>\n

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