{"id":997,"date":"2019-11-06T23:43:48","date_gmt":"2019-11-06T23:43:48","guid":{"rendered":"http:\/\/sites.rutgers.edu\/ilya-raskin-lab\/?page_id=997"},"modified":"2019-11-07T00:13:32","modified_gmt":"2019-11-07T00:13:32","slug":"pablo-kizelsztein","status":"publish","type":"page","link":"https:\/\/sites.rutgers.edu\/ilya-raskin-lab\/pablo-kizelsztein\/","title":{"rendered":"Pablo Kizelsztein, Postdoctoral Associate"},"content":{"rendered":"<h2 style=\"font-family:Trebuchet MS;font-size: 2rem\"><strong>Postdoctoral Studies<\/strong><\/h2>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">My project was the establishment of animal models of neurodegenerative diseases (EAE-Multiple Sclerosis) and the production of liposomes containing antioxidants in order to treat these pathological situations. During my Post Doc I gained experience in the estasblishment of in vivo models (EAE), preparation and characterization of liposomes as well as pharmacokinetic and biodistributions studies.<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Part of the results were published in a patent (FORMULATIONS COMPRISING AN AMPHIPATHIC WEAK BASE FOR TREATMENT OF NEURODEGENERATIVE CONDITIONS) and also papers are in preparation.<\/li>\n<\/ul>\n<h2 style=\"font-family:Trebuchet MS;font-size: 2rem\"><strong>Industry Experience<\/strong><\/h2>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">As a project manager in QBI, I was in charge of the development of a novel <i>in vitro<\/i> neuronal system to express the candidate genes found to be involved in stroke.<br \/>\nThe development of the P19 embryonic carcinoma cell line included: <\/p>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Establishment of growth conditions including medium and serum optimization of the undifferentiated and differentiated cell line.<\/li>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Characterization of the P19 neuronal system by western blot in order to determine the percentage of neurons and glia in the cultures.<\/li>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Establishment of a cell proliferation assay (Alamar blue) for differentiated neurons that can be adapted to HTS (high throughpout screening) in the company.<\/li>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Establishment of an inducible system in P19 neurons in order to express in a controlled manner our cytotoxic genes.<\/li>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Establishment of the RNAi method in P19 and other cell lines in order to determine loss of function of our candidate genes in the respective cell lines.<\/li>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Development of a cytotoxic assay in primary neuronal cell cultures and P19 differentiated neurons in order to test <i>in vitro<\/i> toxicity of a novel lead candidate.<\/li>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Establishment, planning and management of a preliminary <i>in vivo<\/i> toxicity study of a novel lead compound administered to mice.<\/li>\n<\/ul>\n<\/ul>\n<h2 style=\"font-family:Trebuchet MS;font-size: 2rem\"><strong>Ph. D. &#8211; Weizmann Institute of Science<\/strong><\/h2>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">The topic of my thesis was the mechanism of activation and desensitization of the AMPA glutamate receptors.<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Ionotropic glutamate receptors (iGluRs) mediate excitatory synaptic transmission in vertebrates and invertebrates through ligand-induced opening of membrane-embedded receptor channels. These receptors play a crucial role in the development and function of the nervous system, are involved in memory and learning, and are implicated in neurological dysfunctions such as Alzheimer&#8217;s, Parkinson&#8217;s and Huntington&#8217;s disease, schizophrenia, epilepsy, Rasmmusen&#8217;s encephalitis and stroke.<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">My Ph. D. work has been devoted to studies aiming at an understanding of the molecular mechanisms of activation and desensitization of the AMPA receptor GluR1 and the dependence of this processes on the nature of the agonist. The understanding of this mechanism is of extreme importance as it mat contribute to the development of new and better glutamatergic ligands that will affect the pathological conditions in which the ionotropic glutamate receptors are involved.<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">During my Ph.D. studies I gained background and experience in biochemistry, molecular and cell biology, electrophysiology and pharmacology. Prior to my doctoral studies I completed a M.Sc. at the Department of Human Microbiology in the Sackler School of Medicine in Tel Aviv. My thesis topic was the purification of a small GTP binding protein involved in the activation of the NADPH oxidase enzyme complex of the macrophages. Here I gained experience in HPLC purification of proteins, SDS-PAGE and in preparation of polyclonal antibodies.<\/li>\n<\/ul>\n<h2 style=\"font-family:Trebuchet MS;font-size: 2rem\"><strong>Publications<\/strong><\/h2>\n<ol>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\"><strong>Pablo Kizelsztein<\/strong>, and Ilya Raskin. Method of Treating Demyelinating CNS Diseases. US patent application <a href=\"http:\/\/appft.uspto.gov\/netacgi\/nph-Parser?Sect1=PTO2&amp;Sect2=HITOFF&amp;p=1&amp;u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&amp;r=1&amp;f=G&amp;l=50&amp;co1=AND&amp;d=PG01&amp;s1=20100104675.PGNR.&amp;OS=DN\/20100104675&amp;RS=DN\/20100104675\">20100104675<\/a>, 2010.<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\"><strong>Kizelsztein, P.<\/strong>, Govorko, D., Komarnytsky, S., Evans, E., Wang, Zh., Cefalu, W. T., and Raskin, I. 20-Hydroxyecdysone decreases weight and hyperglycemia in a diet-induced obesity mice model. American Journal of Physiology &#8211; Endocrinology and Metabolism, Vol. 296, No. 3, pp. <a href=\"http:\/\/dx.doi.org\/10.1152\/ajpendo.90772.2008\">E433-E439<\/a>, 2009.<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Veronica Wasserman*, <strong>Pablo Kizelsztein<\/strong>*, Olga Garbuzenko, Rony Kohen, Haim Ovadia, Rinat Tabakman and Yechezkel Barenholz. The antioxidant Tempamine: <i>In Vitro<\/i> Antitumor and Neuroprotective Effects and Optimization of Liposomal Encapsulation and Release. Langmuir, 23(4): 1937-1947, 2007.\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">* &#8211; Equal contribution authors.<\/li>\n<\/ul>\n<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\"><strong>Pablo Kizelsztein<\/strong>, Haim Ovadia, Olga Garbuzenko, Alexander Sigal and Yechezquel Barenholz. Tempamine: An amphipathic weak base encapsulated in stable stabilized liposome crosses the blood brain barrier and ameliorates experimental autoimmune encephalomyelitis. In preparation.<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Strutz, N., Villmann, C., Breitinger, H. G., Werner, M., Wenthold, R. J., <strong>Kizelsztein, P.<\/strong>, Teichberg, V. I., and Hollmann, M. Kainate binding proteins (KBPs) are rendered functional ion channels upon transplantation of two short pore-flanking domains from a kainate receptor (2002). J. Biol. Chem. 277 (50) 48035-42<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Strutz, N., Villmann, C., Thalhammer, A., <strong>Kizelsztein, P.<\/strong>, Eisenstein, M., Teichberg, V. I. and Hollmann, M. Localization of Domains and Amino Acids Involved in GluR7 Ion Channel Function (2001).  J. Neurosci. 21 (2), 401-11<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\"><strong>Kizelsztein, P.<\/strong>, Eisenstein, M., Strutz, R., Hollmann, M. and Teichberg, V. I. Mutant cycle analysis of the active and desensitized states of an AMPA receptor induced by Willardiines (2000). Biochemistry 39 (42), 12819-12827.<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\"><strong>Kizelsztein, P.<\/strong>, Eisenstein, M., Strutz, R., Hollmann, M. and Teichberg, V.I. Analysis at atomic resololution of the activated and desensitized state of an AMPA receptor induced by Willardiines (1999). Neurosci. Letters 54, S24 (abstract).<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Everts, I., Petroski, R., <strong>Kizelsztein, P.<\/strong>, Teichberg, V. I., Heinemann, S. F. and Hollmann, M. (1999). Lectin-induced inhibition of desensitization of the kainate receptor GluR6 depends on the activation state and can be mediated by a single native or ectopic N-linked carbohydrate side chain. J. Neurosci. 19 (3), 916-27<\/li>\n<p><\/p>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\"><strong>Kizelsztein, P.<\/strong>, and Teichberg, V. I. (1998). Binding site docking of willardiines in the activation and desensitization of the GluR1 subtype of AMPA receptors. Neurosci. Letters 51, S22 (abstract).<\/li>\n<p><\/ol>\n<p><\/p>\n<p><strong><font face=\"Trebuchet MS\" size=\"3\">Participation at professionals meetings:<\/strong><\/font><\/p>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">January 2006:\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Best student abstract award  selected for oral presentation in the<br \/>\nAnnual meeting of the Israel Society for Free Radical Research at Ben-Gurion University of the Negev. &#8220;Treatment of neurodegenerative diseases by antioxidant loaded sterically stabilized liposomes&#8221;.<\/li>\n<p>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">October 2004:\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Poster presentation in a workshop entitled &#8220;Brain uptake and utilization of fatty acids, lipids and lipoproteins&#8221; (applications to neurological disorders), Bethesda &#8211; Maryland.<\/li>\n<p><\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">September 2004:\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Poster presentation in the 5<sup>th<\/sup> Annual meeting of the Israeli chapter of controlled release society (ICRS).<\/li>\n<p><\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">March 2001:\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Participation in the American College of Cardiology (ACC 50) Annual Scientific Session Orlando, Florida.<\/li>\n<p><\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">December 2000:\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Poster presentation at the Israeli Society for Neuroscience meeting in Eilat, Israel.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">December 1998:\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Participant at the annual meeting of the Israeli Society for Neuroscience.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">November 1998:\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Poster presentation in a workshop entitled &#8220;Structure and Function of Ion Channels&#8221;, in Madrid, Spain.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p><\/p>\n<h2 style=\"font-family:Trebuchet MS;font-size: 2rem\"><strong>Technical Skills<\/strong><\/h2>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Receptor binding assays, protein purification, enzyme assays, HPLC, SDS-PAGE, Western blotting, preparation of polyclonal antibodies, expression of genes in mammalian cells in culture, neuronal cell culture, <i>Xenopus<\/i> oocyte analysis, electrophysiology, two electrode voltage clamp measurements, recombinant DNA techniques, site-directed mutagenesis and protein expression in <i>E. coli<\/i>, bioinformatics and biomedical analysis. <i>In vivo<\/i> toxicological studies, pharmacokinetic and biodistribution in animal studies.<\/li>\n<\/ul>\n<h2 style=\"font-family:Trebuchet MS;font-size: 2rem\"><strong>Computer Skills<\/strong><\/h2>\n<ul>\n<li style=\"font-family:Trebuchet MS;font-size: 1rem\">Microsoft Office applications (Word, Excel, PowerPoint), Photoshop, Canvas, PS8 project management software, SigmaPlot, Cricketgraph, Prisma Graphpad software. Macintosh and PC proficient.<\/li>\n<\/ul>\n<p><\/font><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Postdoctoral Studies My project was the establishment of animal models of neurodegenerative diseases (EAE-Multiple Sclerosis) and the production of liposomes containing antioxidants in order to treat these pathological situations. During &hellip; <a href=\"https:\/\/sites.rutgers.edu\/ilya-raskin-lab\/pablo-kizelsztein\/\" class=\"\">Read More<\/a><\/p>\n","protected":false},"author":82,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-997","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Pablo Kizelsztein, Postdoctoral Associate - Ilya Raskin&#039;s Laboratory<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.rutgers.edu\/ilya-raskin-lab\/pablo-kizelsztein\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Pablo Kizelsztein, Postdoctoral Associate - Ilya Raskin&#039;s Laboratory\" \/>\n<meta property=\"og:description\" content=\"Postdoctoral Studies My project was the establishment of animal models of neurodegenerative diseases (EAE-Multiple Sclerosis) and the production of liposomes containing antioxidants in order to treat these pathological situations. 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