{"id":386,"date":"2019-05-09T17:03:13","date_gmt":"2019-05-09T17:03:13","guid":{"rendered":"http:\/\/sites.rutgers.edu\/james-white-laboratory\/?page_id=386"},"modified":"2022-10-02T05:50:23","modified_gmt":"2022-10-02T05:50:23","slug":"protocols","status":"publish","type":"page","link":"https:\/\/sites.rutgers.edu\/james-white-laboratory\/protocols\/","title":{"rendered":"Protocols"},"content":{"rendered":"

These protocols are frequently used in our lab. Many are written from our own work and some are well established in the scientific community. All protocols are subject to review by professors or other researchers who are experts on each topic before posting.<\/p>\n

Some protocols are written specifically for people and equipment specific to our department. In most cases, a link to the equipment manual is provided to help researchers using the protocol off-site.<\/p>\n

\n

<\/p>\n

\n
Media<\/a><\/h5>\n<\/div>\n
Recipes and usage information on different types of media commonly used in the lab<\/div>\n

<\/p>\n

\n
Seed Sterilization<\/a><\/h5>\n<\/div>\n
Information on how to sterilize certain types of seeds used in the lab<\/div>\n

<\/p>\n

\n
-80C Storage<\/a><\/h5>\n<\/div>\n
Steps for getting microbes ready for storage in the -80C freezer<\/div>\n

<\/p>\n

\n
PCR<\/a><\/h5>\n<\/div>\n
Protocol for carrying out a PCR + gel electrophoresis<\/div>\n

<\/p>\n

\n
Quantitative Real-Time PCR (qRT-PCR)<\/a><\/h5>\n<\/div>\n
Protocol for carrying out a qRT-PCR<\/div>\n

<\/p>\n

\n
Sequencing<\/a><\/h5>\n<\/div>\n
How to prep DNA samples for sequencing<\/div>\n

<\/p>\n

\n
Phylogenetic Tree Construction<\/a><\/h5>\n<\/div>\n
Tutorial for creating a phylogenetic tree using a DNA sequence<\/div>\n

<\/p>\n

\n
Waste<\/a><\/h5>\n<\/div>\n
Proper disposal protocols for waste<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

These protocols are frequently used in our lab. Many are written from our own work and some are well established in the scientific community. All protocols are subject to review … Read More<\/a><\/p>\n","protected":false},"author":242,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-custom.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-386","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nProtocols - James White Laboratory<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.rutgers.edu\/james-white-laboratory\/protocols\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Protocols - James White Laboratory\" \/>\n<meta property=\"og:description\" content=\"These protocols are frequently used in our lab. Many are written from our own work and some are well established in the scientific community. 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