{"id":365,"date":"2021-03-29T15:00:57","date_gmt":"2021-03-29T15:00:57","guid":{"rendered":"http:\/\/sites.rutgers.edu\/jin-lab\/?page_id=365"},"modified":"2021-06-24T20:54:58","modified_gmt":"2021-06-24T20:54:58","slug":"research","status":"publish","type":"page","link":"https:\/\/sites.rutgers.edu\/jin-lab\/research\/","title":{"rendered":"Research"},"content":{"rendered":"

1. Next-generation CRISPR technologies: Developing Therapeutic Base Editing Agents for Genetic Diseases and Cancer.<\/h4>\n

 <\/p>\n

Over 6,000 human genetic diseases are caused by a point mutation. Recent development in CRISPR technology generates hope for a cure for those diseases. Current CRISPR- based gene-editing approaches rely on generating double-strand DNA breaks (DSBs). DSBs can either induce non-homologous end joining (NHEJ) to generate a frameshift mutation to inactivate the target gene or trigger homology-dependent repair (HDR) to correct a mutation. Unfortunately, these approaches have two main limitations: somatic cells do not have HDR activity or have very low HDR activity, and DNA double-strand breaks are highly oncogenic.<\/p>\n

Base editing is a precision genome technology that can introduce targeted nucleotide modifications without the need to generate DSB at the target site. This is achieved by the recruitment of a DNA deaminase, such as a cytidine deaminase, to a nuclease-deficient Cas9\/sgRNA complex. Pin-point\u2122<\/a> (Fig. 1) is a modular base editing system in which the effector and Cas9 are separate molecules. The effector module is recruited to the CRISPR\/sgRNA complex by a chimeric RNA molecule containing an RNA aptamer (Fig. 2). Pin-point, invented in 2015, can be used to correct a loss of function mutation or install premature stop codons for therapeutic gene targeting (knockout) (Fig. 3) (Collantes et al., 2021<\/strong>).<\/p>\n","protected":false},"excerpt":{"rendered":"

1. Next-generation CRISPR technologies: Developing Therapeutic Base Editing Agents for Genetic Diseases and Cancer.   Over 6,000 human genetic diseases are caused by a point mutation. Recent development in CRISPR … Read More<\/a><\/p>\n","protected":false},"author":21,"featured_media":601,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-custom.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-365","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"\nResearch - Victor Shengkan Jin Laboratory<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.rutgers.edu\/jin-lab\/research\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Research - Victor Shengkan Jin Laboratory\" \/>\n<meta property=\"og:description\" content=\"1. 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