{"id":548,"date":"2020-01-30T21:05:15","date_gmt":"2020-01-30T21:05:15","guid":{"rendered":"http:\/\/sites.rutgers.edu\/kohn-lab\/?page_id=548"},"modified":"2020-05-26T16:38:42","modified_gmt":"2020-05-26T16:38:42","slug":"biological-characterization","status":"publish","type":"page","link":"https:\/\/sites.rutgers.edu\/lbr\/biological-characterization\/","title":{"rendered":"Biological Characterization"},"content":{"rendered":"

Biological Characterization<\/b><\/h4>\n

Four (4) Baker SterilGARD II Biosafety cabinets<\/strong> – <\/span>Laminar flow hoods are available for tissue culture and handling to ensure optimal sterility in all cultures and are equipped with gas and vacuum lines. These hoods are BSL 2 certified.\u00a0<\/span><\/p>\n

Thermocycler and Roche Lightcycler 480<\/strong> – <\/span>This instrument is used for quantitative PCR analysis.\u00a0<\/span><\/p>\n

Cell culture incubators<\/strong> – <\/span>Three\u00a0 incubators with temperature, CO<\/span>2<\/span> and humidity controls are for standard cell- and tissue-culturing.\u00a0 One cell culture incubator is adapted to accommodate bioreactor set-up.<\/span><\/p>\n

Temperature\/ humidity stability chamber<\/strong>\u00a0– <\/span>The stability chamber has controlled temperature and humidity environments for use in shelf life testing of polymers, nutraceutical and pharmaceutical products and packaging.\u00a0<\/span><\/p>\n

Refrigerated tabletop centrifuge<\/strong> – <\/span>This centrifuge is used for\u00a0 cell and sample preparation under refrigerated condition.\u00a0\u00a0<\/span><\/p>\n

TECAN Spark plate reader<\/strong> – <\/span>TECAN Spark plate reader measures the fluorescence or the absorbance of samples in 24-well, 48-well and 96-well formats. This is commonly used for assays including alamar blue and pico green.\u00a0<\/span><\/p>\n

Nexcelom Bioscience Cellometer Auto T4<\/strong> – <\/span>Cellometer is used to count cell numbers automatically<\/span><\/p>\n

Power Wave Absorbance Plate Reader<\/strong> (Bio-Tech Instruments) – <\/span>The Power Wave Absorbance Plate Reader (Bio-Tech Instruments) is available for nucleic acid\/protein quantification as well as fluorescence\/luminescence assays.<\/span><\/p>\n

Leica CM1850 Cryostat<\/strong> – <\/span>The Leica CM1850 Cryostat is available to make tissue sections for histological staining or immunofluorescent staining.<\/span><\/p>\n

Horizontal gel electrophoresis apparatus<\/strong> – <\/span>The Horizontal gel electrophoresis apparatus for DNA and RNA analysis.<\/span><\/p>\n

BioRad Mini-PROTEAN\u00ae<\/strong> electrophoresis and transfer system<\/strong> – <\/span>The BioRad Mini-PROTEAN<\/span>\u00ae<\/span> electrophoresis and transfer system is used for SDS-PAGE and Western Blotting needs.<\/span><\/p>\n

\"\" \"\" \"\" \"\" \"\"\u00a0<\/span>\"\"\u00a0\"\" \"\"<\/span><\/p>\n

\n

Microscopes<\/strong><\/h4>\n

Olympus inverted and reflected light microscopes<\/strong> – <\/span>Olympus inverted and reflected light microscopes are available for monitoring cell viability and culture growth. An imaging system equipped with a digital camera and an adaptor is connected to the inverted microscope and enables histological examination and capturing important cell behaviors on different substrates.<\/span><\/p>\n

Zeiss Axio Observer D1 inverted epifluorescent microscope<\/strong> –\u00a0equipped with 4 filters for detecting DAPI, GFP, Rhodamine and Far-red fluorescent samples – <\/span>Our Zeiss Axio Observer D1 inverted epifluorescent microscope is equipped with 4 filters for detecting DAPI, GFP, Rhodamine and Far-red fluorescent samples.\u00a0<\/span><\/p>\n

Confocal Laser Scanning Microscope<\/strong> – <\/span>Confocal microscopy <\/span>is for high resolution and 3-dimensional imaging of samples.\u00a0 It is equipped with an environmental chamber for time-lapse imaging.<\/span><\/p>\n

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Biological Characterization Four (4) Baker SterilGARD II Biosafety cabinets – Laminar flow hoods are available for tissue culture and handling to ensure optimal sterility in all cultures and are equipped … Read More<\/a><\/p>\n","protected":false},"author":801,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-548","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nBiological Characterization - Laboratory for Biomaterials Research<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.rutgers.edu\/lbr\/biological-characterization\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Biological Characterization - Laboratory for Biomaterials Research\" \/>\n<meta property=\"og:description\" content=\"Biological Characterization Four (4) Baker SterilGARD II Biosafety cabinets – Laminar flow hoods are available for tissue culture and handling to ensure optimal sterility in all cultures and are equipped … Read More\" \/>\n<meta property=\"og:url\" content=\"https:\/\/sites.rutgers.edu\/lbr\/biological-characterization\/\" \/>\n<meta property=\"og:site_name\" content=\"Laboratory for Biomaterials Research\" \/>\n<meta property=\"article:modified_time\" content=\"2020-05-26T16:38:42+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/sites.rutgers.edu\/kohn-lab\/wp-content\/uploads\/sites\/382\/2020\/05\/Biosafety-cabinets-1-150x150.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/sites.rutgers.edu\/lbr\/biological-characterization\/\",\"url\":\"https:\/\/sites.rutgers.edu\/lbr\/biological-characterization\/\",\"name\":\"Biological Characterization - 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