Publications

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FRET TENSION PROBE / FLIM-FRET / CELLULAR MECHANICS:

Förster resonant energy transfer efficiency measurements on vinculin tension sensors at focal adhesions using a simple and cost-effective setup, C. Dubois, L. Houel-Renault, M. Erard, N.N. Boustany, N. Westbrook*, J. Biomedical Optics, In Press (2023)

Förster Resonance Energy Transfer Efficiency of the Vinculin Tension Sensor in Cultured Primary Cortical Neuronal Growth Cones, M.A. Ayad, T. Mahon, M.M Cararo-Lopes, M. Patel, I. Hacihaliloglu, B.L. Firestein, N.N. Boustany, Neurophotonics, 9(2), 025002 (2022)  https://doi.org/10.1117/1.NPh.9.2.025002

Linear behavior of the phase lifetime in frequency-domain fluorescence lifetime imaging of FRET constructs, D. D. Sumetsky, J.Y. Jiang, M.A. Ayad, T. Mahon, A. Menaesse,  M.M. Cararo-Lopes, M. Patel, B.L. Firestein, N.N. Boustany, Frontiers in Physics, 9(242), 648016 (2021) https://doi.org/10.3389/fphy.2021.648016

Simplified Instrument Calibration for Wide-Field Fluorescence Resonance Energy transfer (FRET) measured with the Sensitized Emission Method, A. Menaesse, D. Sumetsky, N. Emanuely, J.L. Stein, E.M. Gates, B.D. Hoffman, N.N. Boustany, Cytometry Part A, 99A: 407–416, (2021) (First published: 22 July 2020) https://doi.org/10.1002/cyto.a.24194

FRET Efficiency Measurement in a Molecular Tension Probe with a Low-Cost Frequency-Domain Fluorescence Lifetime Imaging Microscope , J-P Dumas, J.Y. Jiang, E.M. Gates, B.D. Hoffman, M.C. Pierce, N.N. Boustany, J. Biomedical Optics, , 24(12), 126501 (2019) DOI: 10.1117/1.JBO.24.12.126501

      CONFERENCE PROCEEDINGS AND ABSTRACTS:

Combining FRET tension sensor and optical tweezer for mechanical studies on living cells, C. Dubois, L. Houel-Renault, M. Erard, N.N. Boustany, N. Westbook, 26ème Congrès Général de la SFP, 2023.

Feasibility of imaging intracellular tension probes in multicellular aggregates, L. Hu, J. Ong, M. Barroso, and N.N. Boustany*, in Proceedings of the SPIE, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XIV; Volume 12398, 1239804 (2023). https://doi.org/10.1117/12.2655401

Automated Segmentation of Fluorescent Neurons using Deep Learning, M.A. Ayad, I. Hacihaliloglu, T. Mahon, M. M. Cararo-Lopes, B. L. Firestein, and N. N. Boustany, Annual Meeting of the Biomedical Engineering Society, San Antonio, TX, Oct. 2022.

Force measurement on adherent living cells by FRET fluorescence microscopy, C. Dubois, C. Hecquet, L. Houel-Renault, M. Erard, N.N. Boustany, N. Westbook, ICO-25 & OWLS-16, 2022.

Measuring FRET efficiency with phase lifetime, D. D. Sumetsky, J.Y. Jiang, M.A. Ayad, T. Mahon, A. Menaesse,  M.M. Cararo-Lopes, M. Patel, B.L. Firestein, N.N. Boustany, Biomedical Optics Congress, OPTICA, Ft. Lauderdale, FL, April 2022.

Biophysical measurements of subcellular dynamics, Nada N. Boustany, SPIE Photonics West BiOS Symposium, San Francisco, CA, Jan. 2022 (Invited)

Vinculin Tension Probe in Neurons, M. Ayad, T. Mahon, D. Sumetsky, M.M. Cararo-Lopes, B.L. Firestein and N.N. Boustany, in Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; Editor(s): Victor X. D. Yang, Jana M. Kainerstorfer, Qingming Luo, Jun Ding, Ling Fu, Samarendra K. Mohanty, Anna W. Roe, Shy Shoham, Proceedings of the SPIE, Volume 11629, 1162921 (2021).

Simplified Instrument Calibration for FRET Microscopy, A. Menaesse, D. Sumetsky, E.M. Gates, B.D. Hoffman, N.N. Boustany, Annual Meeting of the Biomedical Engineering Society, Virtual, Oct. 2020.

Apoptosis Resistance and Changes in Tension at Focal Adhesions, T. Mahon, A. Menaesse, M. Ayad, D. Sumetsky, N. Emanuely, N.N. Boustany, Annual Meeting of the Biomedical Engineering Society, Virtual, Oct. 2020.

FRET Efficiency Measurement with a Low-Cost Fluorescence Lifetime Imaging Microscope, JP Dumas, J. Jiang, E.M. Gates, B.D. Hoffman, M.C. Pierce, N.N. Boustany, Biomedical Optics Congress, OSA Technical Digest (Optical Society of America, 2020)

An Investigation of a Vinculin Tension Probe within Isolated Cortical Neurons, J. Stein, D. Sumetsky, N. Emanuely, M. Patel, M.M. Cararo-Lopes, B.L. Firestein and N.N. Boustany, Annual Meeting of the Biomedical Engineering Society, Philadelphia, PA, Oct. 2019.

Characterizing the Endogenous Neuronal Distribution of Vinculin for an Investigation of the Forces that Transduce Mechanical Cues in Neurons, E. Kelly and N.N. Boustany, Annual Meeting of the Biomedical Engineering Society, Philadelphia, PA, Oct. 2019.

Calibration and Measurement of FRET Efficiency in a Vinculin Tension Probe, J. Stein, D. Sumetsky, N. Emanuely, M. Patel, M. Cararo Lopes, B. Firestein, N. Boustany, Abstract 1B-3, 45^th Northeast Bioengineering Conference, Rutgers University, Piscataway, NJ, Mar. 2019.

FRET based biosensors in iBMK cells to measure FRET efficiency in fluorescent vinculin tension sensing probes, N. Almodovar, N.N. Boustany, Annual Meeting of the Biomedical Engineering Society, Atlanta, GA, Oct. 2018.

 

BRAIN-ON-A-CHIP DEVICE / NEURONAL INJURY / MITOCHONDRIA:

Glutamate Receptors Mediate Changes to Dendritic Mitochondria in Neurons Grown on Stiff Substrates, S. Kumarapuram, A.J. Kunnath, A. Omelchenko, N.N. Boustany, and B.L. Firestein*, Annals BME, 50: 1116-1133 (2022). https://doi.org/10.1007/s10439-022-02987-0

Dynamin and reverse-mode sodium calcium exchanger blockade confers neuroprotection from diffuse axonal injury , A. Omelchenko, A. Shirao, A. Bhattiprolu, J.D. Zahn, R. S. Schloss, S. Dickson, D.F. Meaney, N.N. Boustany, M.L. Yarmush, and B.L. Firestein, Cell Death and Disease, Vol. 10, Article Number 727 (2019) https://doi.org/10.1038/s41419-019-1908-3

Microfluidic platforms for the study of neuronal injury in vitro, A.B. Shirao, F.H. Kung, A. Omelchenko, R.S. Schloss, N.N. Boustany, J.D. Zahn, M.L. Yarmush, B.L. Firestein, Biotechnology Bioeng, 115(4): 815-830 (2018) doi: 10.1002/bit.26519

 

ZINC OXIDE QCM BIO-SENSOR:

ZnO Nanostructure-Modified QCM for Dynamic Monitoring of Cell Adhesion and Proliferation, P.I. Reyes, Z. Duan, and Y. Lu, D. Khavulya and N. Boustany, Biosensors and Bioelectronics, 41: 84-89, (2013). doi:10.1016/j.bios.2012.07.039

Zinc oxide-based nanostructure modified QCM for dynamic monitoring of cell adhesion and proliferation, Y. Lu, P.I. Reyes, and N.N. Boustany, US Patent # 8,377,683 B2, Feb. 19 2013.

A ZnO Nanostructure-Based Quartz Crystal Microbalance Device for Biochemical Sensing, P.I. Reyes, Z. Zhang, HH Chen, ZQ Duan, J. Zhong, G. Saraf, YC Lu, O. Taratula, E. Galoppini, N.N. Boustany, IEEE Sensors Journal, 9 (10), 1302-1307 (2009).  doi:10.1109/JSEN.2009.2030250  pdf

      CONFERENCE PROCEEDINGS AND ABSTRACTS:

Multifunctional ZnO-Based Thin-Film Bulk Acoustic Resonator for Biosensor Applications, P. I. Reyes, Y. Chen, Z. Duan, G. Saraf, R. Wittstruck, Y. Lu, O. Taratula, E. Galoppini, and N.N. Boustany, Proceedings of the US Workshop on the Physics and Chemistry of II-IV Materials, Las Vegas, NV, November 11-13, 2008.

ZnO Nanotip-based QCM Sensors, P. I. Reyes, Z. Zhang, Z. Duan, J. Zhong, and Y. Lu, O. Taratula, E. Galoppini, and N.N. Boustany, Proceedings of the Electronic Materials Conference (EMC 2008), University of California, Santa Barbara, CA, June 25-27, 2008.

 

OPTICAL SCATTER IMAGING / MITOCHONDRIA / APOPTOSIS:

Label-Free Dynamic Segmentation and Morphological Analysis of Subcellular Optical Scatterers, M. Naser, R. Schloss, P. Berjaud, and N.N. Boustany, Journal of Biomedical Optics, 23(9), 096004 (2018). https://doi.org/10.1117/1.JBO.23.9.096004

Label-free classification of Bax/Bak expressing vs. double-knockout cells, M. Naser, M.T. Graham, K. Pierre, N.N. Boustany, Annals BME, 44(11):3398-3407, (2016). https://doi.org/10.1007/s10439-016-1649-8

Light Scatter Spectroscopy and Imaging of Cellular and Subcellular Events, N.N. Boustany and N.V. Thakor, in Biomedical Photonics Handbook, Second Ed., Volume 1: Fundamentals, Devices and Techniques, Editor: Tuan Vo-Dinh, Ph.D., CRC press, Boca Raton, FL, Chapter 14, p. 461-488, (2015).

Measurement of object structure from size-encoded images generated by optically implemented Gabor filters, H. Sierra, J-Y. Zheng, B. Rabin and N.N. Boustany, Optics Express, 20 (27), 28698-28706 (2012). https://doi.org/10.1364/OE.20.028698

Detection of mitochondrial fission with orientation-dependent optical Fourier filters, R.M. Pasternack, J-Y. Zheng, N.N. Boustany, Cytometry Part A. 79A: 137-148 (2011). doi: 10.1002/cyto.a.21011 The results of this article were selected for inclusion in the “In This Issue” features. pdf

Quantifying subcellular dynamics in apoptotic cells with optical Gabor-like filtering , R.M. Pasternack, B. Rabin, J-Y. Zheng and N.N. Boustany, Biomedical Optics Express, 1 (2), 720–728 (2010). (invited). doi:10.1364/BOE.1.000720 pdf

Optical Scatter Changes at the Onset of Apoptosis Are Spatially Associated with Mitochondria, R.M. Pasternack, J-Y. Zheng, N.N. Boustany, Journal of Biomedical Optics Letters. 15 (4), 040504 (2010). doi: 10.1117/1.3467501  This article was selected for the Sep. 1 2010 issue of Virtual Journal of Biological Physics Research. pdf

Alterations in the characteristic size distributions of subcellular scatterers at the onset of apoptosis: effect of Bcl-x_L and Bax/Bak , J-Y. Zheng and N.N. Boustany, J. Biomedical Optics, 15 (4), 045002 (2010). doi: 10.1117/1.3462933 This article was selected for the Aug. 1 2010 issue of Virtual Journal of Biological Physics Research. pdf

Microscopic Imaging and Spectroscopy with Scattered Light , N.N. Boustany, S.A. Boppart, V. Backman, Annual Review of Biomedical Engineering, Volume 12, In press (2010). (invited) pdf  https://doi.org/10.1146/annurev-bioeng-061008-124811

Optical scatter microscopy based on two-dimensional Gabor filters, N.N. Boustany, R.M. Pasternack, J-Y. Zheng Journal of Visualized Experiments, http://www.jove.com/index/details.stp?id=1915, (2010) doi:10.3791/1915   pdf, video

Optical scatter imaging with a digital micromirror device, J-Y. Zheng, R.M. Pasternack, N.N. Boustany, Optics Express, 17 (22), 20401-20414 (2009).  doi:10.1364/OE.17.020401   pdf

Highly sensitive size discrimination of submicron objects using optical Fourier filtering based on two-dimensional Gabor filters , R.M. Pasternack, Z. Qian, J-Y. Zheng, D.N. Metaxas, N.N. Boustany, Optics Express, 17(14), 12001-12012 (2009). On Aug. 7 2009, results of this article were featured on the Technical Insights, Sensor Technology Alert Services, of the Frost & Sullivan Website.  doi:10.1364/OE.17.012001  pdf

Measurement of Subcellular Texture by Optical Gabor-Like Filtering with a Digitial Micromirror Device, R.M. Pasternack, Z. Qian, J-Y. Zheng, D.N. Metaxas, E. White, N.N. Boustany, Optics Letters, 33(19), 2209-2211 (2008). Erratum in Optics Letters, 34(13), 1939 (2009).This article was selected for the Nov. 1 2008 issue of Virtual Journal of Biological Physics Research, and for the Dec. 2008  issue (Vol 3 Issue 12) of The Virtual Journal for Biomedical Optics. https://doi.org/10.1364/OL.33.002209 erratum: https://doi.org/10.1364/OL.34.001939 pdf

The C-terminal transmembrane domain of Bcl-x_L mediates changes in mitochondrial morphology, J-Y. Zheng, Y.C. Tsai, P. Kadimcherla, R. Zhang, J. Shi, G.A. Oyler, N.N. Boustany, Biophysical J., 94 (1): 286-297 (2008). doi:10.1529/biophysj.107.104323  pdf

Bcl-x_L-dependent light scattering by apoptotic cells, N.N. Boustany, Y.C. Tsai, G.A. Oyler, B. Pfister, W.M. Joiner, N.V. Thakor, Biophysical J., 87 (6): 4163-4171 (2004). doi:10.1529/biophysj.104.048736     pdf

Light Scatter Spectroscopy and Imaging of Cellular and Subcellular Events, N.N. Boustany and N.V. Thakor, in Biomedical Photonics Handbook, Editor: Tuan Vo-Dinh, Ph.D., CRC press, Boca Raton, FL, p. 16.1-16.23, (2003).  pdf

Calcium-induced alterations in mitochondrial morphology quantified in situ with Optical Scatter Imaging, N.N. Boustany, R. Drezek, N.V. Thakor, Biophysical J., 83: 1691-1700 (2002). The results of this article were featured in the Nov. 2002 issue of Biophotonics International magazine. doi:10.1016/S0006-3495(02)73937-4  pdf

Optical Scatter Imaging: subcellular morphometry in situ with Fourier filtering, N.N. Boustany, S.C. Kuo, N.V. Thakor, Optics Letters, 26(14): 1063-1065 (2001).  The results of this article were featured in the Optics in 2001 highlights in the Dec. 2001 issue of Optics and Photonics News magazine.  doi:10.1364/OL.26.001063  pdf

Optical Scatter Imaging, N.N. Boustany, S.C. Kuo and N.V. Thakor, US Provisional patent # 60,268,374 filed Feb. 13 2001.

      CONFERENCE PROCEEDINGS AND ABSTRACTS:

M. Naser, E. Kelly, C. Ditmars, R.S. Schloss, B.L Firestein and N.N. Boustany, “Fourier-filtering Based Size-Encoded Images for Label-free Tracking of Subcellular Organelles in Neurons, in Biomedical Applications of Light Scattering, Edited by V. Backman and A. Wax, Proceedings of the SPIE, In Press, Jan. 2019.

M. Naser, R.S. Schloss, N.N. Boustany, “Label-free Fourier Filtered Dark-field Imaging to Quantify Subcellular Dynamics,” in Biomedical Applications of Light Scattering, Edited by V. Backman and A. Wax, Proceedings of the SPIE, Jan. 2018.

M. Naser, M.T. Graham, K. Pierre, J.A. Hostettler, H. Sierra, and N.N. Boustany, “Quantification of Bax/Bak-Dependent Morphological Changes by Textural Analysis of Optical Scatter Images” Biomedical Optics, OSA Technical Digest (Optical Society of America, 2014), paper BT3A.37.

N.N. Boustany and H. Sierra “Analyzing subcellular structure with optical Fourier processing based on Gabor filters”, in Biomedical Applications of Light Scattering, Edited by V. Backman and A. Wax, Proceedings of the SPIE, Vol. 8592, Article Number: 85920Q, Jan. 2013. (invited)

H. Sierra and N.N. Boustany, “Characterizing Intracellular Structural Dynamics with Optical Gabor Filtering” Biomedical Optics, OSA Technical Digest (Optical Society of America, 2012), paper BW2B.7.

N.N. Boustany, H. Sierra, R.M. Pasternack, B. Rabin, “Direct and highly sensitive measurement of the spatial arrangement of micro-structures within biological samples”, in Biomedical Applications of Light Scattering, Edited by V. Backman and A. Wax, Proceedings of the SPIE, Vol. 8230, Article Number: 82300O, Jan. 2012.

H. Sierra, R.M Pasternack, B. Rabin, S.N. Masand, J-Y. Zheng, N.N. Boustany, “Optimizing Filter Design for Optical Scatter Microscopy,” Optics in the Life Sciences: OSA Optics and Photonics Congress, Monterey, CA, Apr. 4-6, 2011.

R.M. Pasternack, J-Y. Zheng and N.N. Boustany, “Quantifying mitochondrial dynamics in apoptotic cells with optical Gabor-like filtering”, in Biomedical Optics, OSA Technical Digest (Optical Society of America, 2010), paper BTuE3.

R.M. Pasternack, J-Y Zheng, Z. Qian, J. Zhou, D.N. Metaxas, E. White, and N.N. Boustany, “Optical Processing of Cell and Tissue Structure”, Workshop on Microscopic Image Analysis with Applications in Biology (MIAAB2009), NIH Campus, Bethesda, MD, Sep 3-4 2009.

N.N. Boustany, J-Y. Zheng, R.M. Pasternack, Z. Qian, “Optical Fourier processing microscope to quantify subcellular structure and dynamics”, IEEE International Conference on Complex Medical Engineering, Tempe, AZ April 9-11 2009. ICME INTERNATIONAL CONFERENCE ON COMPLEX MEDICAL ENGINEERING: 395-400 2009.

R.M. Pasternack, Z. Qian, J-Y. Zheng, and N.N. Boustany, “Measurement of subcellular morphology by light scatter filtering with a digital micromirror device,” in Biomedical Applications of Light Scattering, Proceedings of the SPIE, Vol. 7187, Jan. 2009.

J-Y. Zheng, , Z. Qian, R.M. Pasternack and N.N. Boustany, “Microscopy with spatial filtering for sorting particles and monitoring subcellular morphology,” in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues , Proceedings of the SPIE, Vol. 7182, Jan. 2009

N.N. Boustany, J-Y. Zheng, R.M. Pasternack, Z. Qian, “Optical Fourier Processing of Subcellular Structure”, in Laser Science XXIV, OSA Technical Digest (Optical Society of America, 2008), paper LThD2. (invited)

N.N. Boustany “Optical scatter imaging: a microscopic modality for rapid morphological assay of living cells”, in Multimodal Biomedical Imaging, Proceedings of the SPIE, Vol. 6431, Jan. 2007. (invited)

C. Sui and N.N. Boustany, “Potential FDTD application in studying mitochondrial apoptosis” in Biomedical Applications of Light Scattering, Proceedings of the SPIE, Vol. 6446, Jan. 2007.

N.N. Boustany and J-Y. Zheng, “Effect of BCL-2 family proteins on subcellular particle size distribution”, in Biomedical Applications of Light Scattering, Proceedings of the SPIE, Vol. 6446, Jan. 2007.

J-Y. Zheng and N.N. Boustany, “Direct measurement of particle diameter distributions in dark-field microscopy”, in Biomedical Optics, Technical Digest (Optical Society of America, 2006), paper TuI54.

N.N. Boustany, Y.C. Tsai, B. Pfister, W.M. Joiner, G.A. Oyler, N.V. Thakor, “Light scattering by apoptotic cells”, in Frontiers in Optics, OSA Technical Digest (Optical Society of America, 2003), paper WZ1.

W.M. Joiner, N.N. Boustany, N.V. Thakor, “Optical Scatter Imaging of programmed cell death”, Proceedings of the IEEE 28^th Annual Northeast Bioengineering Conference, p. 127-128, April 20-21 2002.

N.N. Boustany, R. Drezek, N.V. Thakor, “Measurement and calculation of angular scatter change in mitochondria during calcium overload”, in Biomedical Topical Meeting, (Optical Society of America, 2002), paper WB7.

E. Kelly, D. Ditmars, M. Naser, R.S. Schloss, A. Omelchenko, B.L. Firestein, and N.N. Boustany, Tracking Changes in Organelle Morphology using a Light-Scatter-Based Label-Free Imaging Technique, Annual Meeting of the Biomedical Engineering Society, Atlanta, GA, Oct. 2018

M. Naser, R. S. Schloss, B. L. Firestein, and N.N. Boustany, Light-Scatter Based Label-free Assessment of Mitochondrial Remodeling and Fission, Annual Meeting of the Biomedical Engineering Society, Phoenix AZ, Oct. 2017.

M. Naser, R. S. Schloss, A. Shrirao, and N.N. Boustany, Generation of Subcellular Micro-Texture Using Label-free Optical Scatter Imaging, Annual Meeting of the Biomedical Engineering Society, Phoenix AZ, Oct. 2017.

M. Naser, M.T. Graham, K. Pierre, O. Ipaye, N.N. Boustany, “A Hybrid Approach for Label-Free Optical Detection of Genetic Alterations,” Annual Meeting of the Biomedical Engineering Society, Tampa, FL, Oct. 2015.

K. Pierre, I. Ahmed, and N. Boustany, “Label-free Quantification of Neuronal Structural Changes by Optical Scatter Image Analysis,” Annual Meeting of the Biomedical Engineering Society, Tampa, FL, Oct. 2015.

R.M. Pasternack, B. Rabin, N.J. Shah, N.N. Boustany, “Study of mitochondrial dynamics during apoptosis by optical Fourier filtering detection”, Biophysical Society Annual Meeting, Baltimore, MD, Mar. 5-9, 2011.

N.N. Boustany, “Quantifying Cellular Function and Dynamics with Light”, 11^th Subtle Technologies Festival, “Light”, Innis Town Hall, University of Toronto, Canada, May 31, 2008.

N.N. Boustany and J-Y. Zheng, “Light-scattering-based morphometric cellular response to transfection by the BCL-x_L transmembrane domain”, International Society for Analytical Cytology XXIII Congress, Abstract # 373, Quebec City, Quebec, Canada, May 20-24 2006.

J-Y. Zheng, Y.C. Tsai, J. Shi, G.A. Oyler, N.N. Boustany, “Transmembrane domain mediates BCL-x_L induced changes in subcellular particle size distributions”, Annual meeting of the Biophysical Society, Salt Lake City, UT, Feb. 2006.

J. Zheng, Y.C. Tsai, J. Shi, G.A. Oyler, N.N. Boustany, “Protein targeting to mitochondria by the BCL-x_L transmembrane domain alters angular light scattering”, Proceedings of the Annual Meeting of the Biomedical Engineering Society, Baltimore, MD, Sep. 2005, Abstract # 904.

N.N. Boustany, “Optical Scatter Imaging of Apoptotic Cells”, The Whitaker Foundation Biomedical Engineering Research Conference Abstracts, p. 115, Aug. 12-14, La Jolla, CA, 2004.

J-Y. Zheng, B. Griffel, Y.C. Tsai, G.A. Oyler, N.N. Boustany, “Characteristic organelle size distributions within apoptosis resistant cells”, Proceedings of the Annual Meeting of the Biomedical Engineering Society, Philadelphia, PA, Oct. 2004, Abstract # 1055.

B. Griffel, J-Y. Zheng, and N.N. Boustany, “Fourier filtering with a Digital Micromirror Device”, presented as part of a forum on undergraduate research at the Annual Meeting of the Biomedical Engineering Society, Philadelphia, PA, Oct. 2004

N.N. Boustany, Y.C. Tsai, B. Pfister, W.M. Joiner, G.A. Oyler, N.V. Thakor, “Optical Scatter Imaging to track mitochondrial alterations during apoptosis”, Cytometry, Supplement 11, p.40, Abstract # 43835, International Society for Analytical Cytology XXI Congress, San Diego, CA, May 4-9 2002.

N.N. Boustany, Y.C. Tsai, B. Pfister, W.M. Joiner, G.A. Oyler, N.V. Thakor, “Optical Scatter Imaging reveals Bcl-x_L-dependent subcellular dynamics at onset of apoptosis”, Biophys. J., 82(1) Part 2, p. 23A-23A, Jan. 2002.

N.N. Boustany, Y.C. Tsai, G.A. Oyler, N.V. Thakor, “In-Situ comparison of mitochondrial light scatter in calcium overload and apoptosis” Biophys. J., 80(1) Part 2, p. 237A, Jan. 2001.

N.N. Boustany, N.V. Thakor, and S.C. Kuo, “Ratiometric imaging in transmission dark-field microscopy for subcellular particle sizing”, Optical Society of America Annual Meeting/ILS XVI Conference Program, p. 67, Abstract MDD5, Providence RI, Oct. 22-26, 2000.

N.N. Boustany, T.L. Thompson, N.V. Thakor, S.C. Kuo, “Dynamic Observation of Organelle Morphology During Calcium Cell Injury with Ratiometric Dark-Field Microscopy”, Annals of Biomedical Engineering, Vol. 28, Suppl. 1, p. S-40, Abstract # T4.58, Biomedical Engineering Society Annual Meeting, Seattle, WA, Oct. 12-16, 2000.

N.N. Boustany, D.S. Gray, S.C. Kuo, N.V. Thakor, “Light Scatter Imaging to Quantify Organelle Size in Live Cells”, Biophys. J., 78(1) Part 2, p 251A-251A, Jan. 2000.

 

UV RESONANCE RAMAN SPECTROSCOPY / CANCER TISSUE ANALYSIS:

Analysis of nucleotides and aromatic amino acids in normal and neoplastic colon mucosa by ultraviolet resonance Raman spectroscopy, N.N. Boustany, J.M. Crawford, R. Manoharan, R. Dasari, M.S. Feld, Laboratory Investigation, 79(10): 1201-1214 (1999). PMID: 10532584 pdf, Abstract

Ultraviolet resonance Raman spectroscopy system of bulk and microscopic human colon tissue, N.N. Boustany, R. Manoharan, R.R. Dasari, M.S. Feld, Applied Spectroscopy, 54(1): 24-30 (2000).  pdf

Absorption coefficient and purine photobleaching rate in colon mucosa during resonance Raman spectroscopy at 251nm, N.N. Boustany, Applied Optics, 40(34): 6396-6405 (2001). doi:10.1364/AO.40.006396  pdf

Effects of freeze-thaw and photobleaching on the ultraviolet resonance Raman spectra of human colon biopsies, N.N. Boustany, J.M. Crawford, R. Manoharan, R.R. Dasari, M.S. Feld, Applied Spectroscopy, 55(11): 1506-1513 (2001).  pdf

     CONFERENCE PROCEEDINGS AND ABSTRACTS:

N.N. Boustany, R. Manoharan, R. Dasari, M.S. Feld, “Analysis of normal and diseased colon mucosa using ultraviolet resonance Raman spectroscopy”, in Advances in Laser and Light Spectroscopy to diagnose Cancer and Other Diseases III: Optical Biopsy, R. R. Alfano Ed., Proceedings of the SPIE, Vol. 2679, p. 66-70, April 1996.

R. Manoharan, Y. Wang, N.N. Boustany, J.F. Brennan III, J.J. Baraga, R.R. Dasari, J. Van Dam, S. Singer, M.S. Feld, “Raman spectroscopy for cancer detection: instrument development and tissue diagnosis”, in Biomedical Optoelectronic Systems and Devices II, N.I Croitoru, N. Kroo, M. Miyagi, R. Pratesi, J.M. Wolfrum, Eds., Proceedings of the SPIE, Vol. 2328, p. 128-132, Dec.1994.

R. Manoharan, N. Boustany, J.F. Brennan III, R.R. Dasari, M.S. Feld, “Biochemical analysis of disease using NIR Raman and UV resonance Raman spectroscopy”, Proceedings of the FACSS, Kansas City, KS, Sep. 29-Oct. 4, 1996.

 

CARTILAGE MECHANICS AND BIOSYNTHESIS:

Time-dependent changes in the response of cartilage to static compression suggest interstitial pH is not the only signaling mechanism, N.N. Boustany, M.L. Gray, A.C. Black, E.B. Hunziker, J. Orthop. Res., 13(5): 740-750 (1995). doi: 10.1002/jor.1100130514

Correlation between synthetic activity and glycosaminoglycan concentration in epiphyseal cartilage raises questions about the regulatory role of interstitial pH, N.N. Boustany, M.L. Gray, A.C. Black, E.B. Hunziker, J. Orthop. Res., 13(5): 733-739 (1995). doi:10.1002/jor.1100130513

      CONFERENCE PROCEEDINGS AND ABSTRACTS:

M.L Gray, A.M. Garcia, N. Boustany, L.M. Lesperance, A. Liu, D. Burstein, “Extracellular matrix charge: measurement by NMR and implications for tissue function, growth, and metabolism”, Plenary Session, Bioelectrical Repair and Growth Society Meeting, Dana Point, CA, Oct. 11-14, 1993.

N.N. Boustany and M.L. Gray, “The regulation of chondrocyte biosynthesis in epiphyseal cartilage: the role of interstitial pH”, Proceedings of the Combined Meeting of the Orthopaedic Research Societies of U.S.A, Japan and Canada, Banff, Alberta, Canada, Oct. 21-23, 1991 (Paper No. 125).

 

DISSERTATIONS:

Characterization of mucosal dysplasia with ultraviolet resonance Raman spectroscopy, Nada N. Boustany, Ph.D. thesis, MIT 1997, Thesis supervisor: Michael S. Feld, Ph.D. (co-advisor: James M. Crawford, MD, Ph.D.)

Regulation of chondrocyte biosynthesis in epiphyseal cartilage: the role of interstitial pH, Nada N. Boustany, S.M. thesis, MIT 1991, Thesis supervisor: Martha L. Gray, Ph.D.

Development of an array of clinically implantable polarographic oxygen sensors, Nada N. Boustany, S.B. thesis, MIT 1988, Thesis supervisor: H. Frederick Bowman, Ph.D.