{"id":1015,"date":"2020-04-22T04:02:38","date_gmt":"2020-04-22T04:02:38","guid":{"rendered":"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/?page_id=1015"},"modified":"2020-04-24T19:55:54","modified_gmt":"2020-04-24T19:55:54","slug":"payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation","status":"publish","type":"page","link":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/","title":{"rendered":"Payne, Paisley: Role of the Neuropeptide OFQ\/N in Neuroinflammation"},"content":{"rendered":"

\"Paisley<\/a><\/p>\n

View video presentation<\/a><\/strong><\/p>\n

Title:<\/strong> Role of the Neuropeptide OFQ\/N in Neuroinflammation<\/p>\n

Name:<\/strong> Paisley Payne<\/p>\n

Major:<\/strong> Biological Sciences and Psychology<\/p>\n

School affiliation:<\/strong> Honors College, School of Arts and Sciences<\/p>\n

Programs:<\/strong> Honors College Capstone, PNI Lab<\/p>\n

Other contributors:<\/strong> Dr. Kusnecov and Marialaina<\/p>\n

Abstract:<\/strong> Bacterial lipopolysaccharide (LPS) is an endotoxin that activates the immunesystem and enables macrophages to release proinflammatory cytokines which induce stress-like effects in the brain. In the current study, the goal was to determine whether the inflammatory and behavioral response to LPS differed in mice deficient for the neuropeptide OFQ\/N, or its receptor, NOP\/ORL. OrphaninFQ\/nociceptin (OFQ\/N) has several functions, and may be important in containing the magnitude of the stress response. Male and female mice with a C57\/BL6 background were grouped by genotype (wild-type, OFQ knockout, or ORL-1 knockout) and treated with an acute injection of LPS (5ug) or saline control. Two hours after injection, mice were tested for behavior in the open field (OF) and responsiveness to a novel object (OF\/NO test). One hour after completion of the 10 minute OF\/NO test, animals were perfused and brains and spleen collected. Spleens were measured for the cytokine interleukin-1\u03b2 (IL-1\u03b2), while brains were assayed for immediate early gene expression (data collection in progress). Wildtype mice responded to LPS by showing delayed entrance into the inner zone surrounding a novel object stimulus. The response of ORL-1 KO mice treated with LPS also showed a significant decrease in distance travelled in the NO\/OF, compared to the saline control. In contrast, for OFQ knockouts there was no significant difference in travel distance between LPS and Saline-treated groups. However, latency to enter the inner zone (i.e. where the novel object was located) was highest for LPS treated OFQ knockouts, and was also significantly different between LPS and saline-treated ORL knockouts. Finally, the IL-1\u03b2 cytokine levels in knockout animals were higher in LPS treated animals compared to saline, confirming the proinflammatory response to LPS. In conclusion, deletion of ORL-1 receptor seems to cause the most significant change in behavior, suggesting that it has a protective function against immune challenge with LPS.<\/p>\n","protected":false},"excerpt":{"rendered":"

View video presentation Title: Role of the Neuropeptide OFQ\/N in Neuroinflammation Name: Paisley Payne Major: Biological Sciences and Psychology School affiliation: Honors College, School of Arts and Sciences Programs: Honors … Read More<\/a><\/p>\n","protected":false},"author":970,"featured_media":0,"parent":441,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-custom.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1015","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nPayne, Paisley: Role of the Neuropeptide OFQ\/N in Neuroinflammation - Senior Exhibits<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Payne, Paisley: Role of the Neuropeptide OFQ\/N in Neuroinflammation - Senior Exhibits\" \/>\n<meta property=\"og:description\" content=\"View video presentation Title: Role of the Neuropeptide OFQ\/N in Neuroinflammation Name: Paisley Payne Major: Biological Sciences and Psychology School affiliation: Honors College, School of Arts and Sciences Programs: Honors … Read More\" \/>\n<meta property=\"og:url\" content=\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/\" \/>\n<meta property=\"og:site_name\" content=\"Senior Exhibits\" \/>\n<meta property=\"article:modified_time\" content=\"2020-04-24T19:55:54+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Payne_Paisley_ArestyPoster_thumbnail-300x225.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/\",\"url\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/\",\"name\":\"Payne, Paisley: Role of the Neuropeptide OFQ\/N in Neuroinflammation - Senior Exhibits\",\"isPartOf\":{\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/#primaryimage\"},\"thumbnailUrl\":\"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Payne_Paisley_ArestyPoster_thumbnail-300x225.png\",\"datePublished\":\"2020-04-22T04:02:38+00:00\",\"dateModified\":\"2020-04-24T19:55:54+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/#primaryimage\",\"url\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Payne_Paisley_ArestyPoster_thumbnail.png\",\"contentUrl\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Payne_Paisley_ArestyPoster_thumbnail.png\",\"width\":1067,\"height\":800,\"caption\":\"Paisley Payne thumbnail\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Our Symposium Partners\",\"item\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Aresty Research Center\",\"item\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"Payne, Paisley: Role of the Neuropeptide OFQ\/N in Neuroinflammation\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/#website\",\"url\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/\",\"name\":\"Senior Exhibits\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Payne, Paisley: Role of the Neuropeptide OFQ\/N in Neuroinflammation - Senior Exhibits","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/","og_locale":"en_US","og_type":"article","og_title":"Payne, Paisley: Role of the Neuropeptide OFQ\/N in Neuroinflammation - Senior Exhibits","og_description":"View video presentation Title: Role of the Neuropeptide OFQ\/N in Neuroinflammation Name: Paisley Payne Major: Biological Sciences and Psychology School affiliation: Honors College, School of Arts and Sciences Programs: Honors … Read More","og_url":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/","og_site_name":"Senior Exhibits","article_modified_time":"2020-04-24T19:55:54+00:00","og_image":[{"url":"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Payne_Paisley_ArestyPoster_thumbnail-300x225.png"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/","url":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/","name":"Payne, Paisley: Role of the Neuropeptide OFQ\/N in Neuroinflammation - Senior Exhibits","isPartOf":{"@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/#website"},"primaryImageOfPage":{"@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/#primaryimage"},"image":{"@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/#primaryimage"},"thumbnailUrl":"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Payne_Paisley_ArestyPoster_thumbnail-300x225.png","datePublished":"2020-04-22T04:02:38+00:00","dateModified":"2020-04-24T19:55:54+00:00","breadcrumb":{"@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/#primaryimage","url":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Payne_Paisley_ArestyPoster_thumbnail.png","contentUrl":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Payne_Paisley_ArestyPoster_thumbnail.png","width":1067,"height":800,"caption":"Paisley Payne thumbnail"},{"@type":"BreadcrumbList","@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/payne-paisley-role-of-the-neuropeptide-ofq-n-in-neuroinflammation\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/"},{"@type":"ListItem","position":2,"name":"Our Symposium Partners","item":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/"},{"@type":"ListItem","position":3,"name":"Aresty Research Center","item":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/"},{"@type":"ListItem","position":4,"name":"Payne, Paisley: Role of the Neuropeptide OFQ\/N in Neuroinflammation"}]},{"@type":"WebSite","@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/#website","url":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/","name":"Senior Exhibits","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/pages\/1015"}],"collection":[{"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/users\/970"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/comments?post=1015"}],"version-history":[{"count":2,"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/pages\/1015\/revisions"}],"predecessor-version":[{"id":1136,"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/pages\/1015\/revisions\/1136"}],"up":[{"embeddable":true,"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/pages\/441"}],"wp:attachment":[{"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/media?parent=1015"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}