{"id":1176,"date":"2020-04-22T19:04:09","date_gmt":"2020-04-22T19:04:09","guid":{"rendered":"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/?page_id=1176"},"modified":"2020-04-23T21:26:48","modified_gmt":"2020-04-23T21:26:48","slug":"abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production","status":"publish","type":"page","link":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/","title":{"rendered":"Abdelhamid, Ahmed: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production"},"content":{"rendered":"<p><a href=\"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-633 alignleft\" src=\"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail-300x169.png\" alt=\"\" width=\"300\" height=\"169\" srcset=\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail-300x169.png 300w, https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail-1024x576.png 1024w, https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail-768x432.png 768w, https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail.png 1422w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><strong><a class=\"btn btn-primary\" href=\"https:\/\/rutgers.box.com\/s\/kigozmc0917ie619dcr5esv7yufjy97q\" target=\"_blank\" rel=\"noopener noreferrer\">View video presentation<\/a><\/strong><\/p>\n<p><strong>Title:<\/strong> Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production<\/p>\n<p><strong>Name:<\/strong> Ahmed Abdelhamid<\/p>\n<p><strong>Major:<\/strong> Biomedical Engineering<\/p>\n<p><strong>School affiliation:<\/strong> School of Engineering<\/p>\n<p><strong>Programs:<\/strong> Aresty &#8211; RA Program<\/p>\n<p><strong>Other contributors:<\/strong> Akshay Thakur, Dharanidaran Jayachandran &amp; Dr. Mohammad Irfan, Dr. Shishir Chundawat<\/p>\n<p><strong>Abstract:<\/strong> The main area of focus in this project is to clone and transform specific inserts of DNA eventually into yeast cells in order to collect their proteins and examine their potential production of polysaccharides. In general, cellular production of polysaccharides from sugar molecules is critical in many metabolic processes to ensure survival of living organisms such as plants, bacteria and even humans. Thus, cell wall polysaccharide synthesis is particularly of interest for biomass and bioenergy applications. There are three main objectives to complete the focus of the project critical for the next step in further research. Firstly, sequence and ligation independent cloning (SLIC) is performed for fusing cellulose synthase (CesA8 from Poplar plant) with fluorescence proteins (Skylan-S and moxMaple3) and is transformed into E.cloni competent cells. This construct is then cloned into a species of yeast named Pichia pastoris for expression. Lastly, the expressed protein is purified using affinity chromatography to get the proteins responsible for polysaccharide synthesis. In terms of further research, the next step is to image these proteins through a proposed multimodal single-molecule optical imaging instrument to study the key aspects of in planta polysaccharide synthesis and their turnover dynamics. The constructs developed here will act as model systems to study the in planta polysaccharide synthesis.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>View video presentation Title: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production Name: Ahmed Abdelhamid Major: Biomedical Engineering School affiliation: School of Engineering Programs: &hellip; <a href=\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/\" class=\"\">Read More<\/a><\/p>\n","protected":false},"author":970,"featured_media":0,"parent":441,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-custom.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1176","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Abdelhamid, Ahmed: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production - Senior Exhibits<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Abdelhamid, Ahmed: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production - Senior Exhibits\" \/>\n<meta property=\"og:description\" content=\"View video presentation Title: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production Name: Ahmed Abdelhamid Major: Biomedical Engineering School affiliation: School of Engineering Programs: &hellip; Read More\" \/>\n<meta property=\"og:url\" content=\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/\" \/>\n<meta property=\"og:site_name\" content=\"Senior Exhibits\" \/>\n<meta property=\"article:modified_time\" content=\"2020-04-23T21:26:48+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail-300x169.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/\",\"url\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/\",\"name\":\"Abdelhamid, Ahmed: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production - Senior Exhibits\",\"isPartOf\":{\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/#primaryimage\"},\"thumbnailUrl\":\"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail-300x169.png\",\"datePublished\":\"2020-04-22T19:04:09+00:00\",\"dateModified\":\"2020-04-23T21:26:48+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/#primaryimage\",\"url\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail.png\",\"contentUrl\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail.png\",\"width\":1422,\"height\":800},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Our Symposium Partners\",\"item\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Aresty Research Center\",\"item\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"Abdelhamid, Ahmed: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/#website\",\"url\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/\",\"name\":\"Senior Exhibits\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Abdelhamid, Ahmed: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production - Senior Exhibits","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/","og_locale":"en_US","og_type":"article","og_title":"Abdelhamid, Ahmed: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production - Senior Exhibits","og_description":"View video presentation Title: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production Name: Ahmed Abdelhamid Major: Biomedical Engineering School affiliation: School of Engineering Programs: &hellip; Read More","og_url":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/","og_site_name":"Senior Exhibits","article_modified_time":"2020-04-23T21:26:48+00:00","og_image":[{"url":"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail-300x169.png"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/","url":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/","name":"Abdelhamid, Ahmed: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production - Senior Exhibits","isPartOf":{"@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/#website"},"primaryImageOfPage":{"@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/#primaryimage"},"image":{"@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/#primaryimage"},"thumbnailUrl":"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail-300x169.png","datePublished":"2020-04-22T19:04:09+00:00","dateModified":"2020-04-23T21:26:48+00:00","breadcrumb":{"@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/#primaryimage","url":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail.png","contentUrl":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/04\/Ahmed_Abdelhamid_thumbnail.png","width":1422,"height":800},{"@type":"BreadcrumbList","@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/abdelhamid-ahmed-cloning-and-expression-of-cellulose-synthase-cesa-in-pichia-pastoris-for-analyzing-cellulose-production\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/"},{"@type":"ListItem","position":2,"name":"Our Symposium Partners","item":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/"},{"@type":"ListItem","position":3,"name":"Aresty Research Center","item":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/"},{"@type":"ListItem","position":4,"name":"Abdelhamid, Ahmed: Cloning and Expression of Cellulose Synthase (CesA) in Pichia pastoris for Analyzing Cellulose Production"}]},{"@type":"WebSite","@id":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/#website","url":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/","name":"Senior Exhibits","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/pages\/1176"}],"collection":[{"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/users\/970"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/comments?post=1176"}],"version-history":[{"count":4,"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/pages\/1176\/revisions"}],"predecessor-version":[{"id":1300,"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/pages\/1176\/revisions\/1300"}],"up":[{"embeddable":true,"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/pages\/441"}],"wp:attachment":[{"href":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-json\/wp\/v2\/media?parent=1176"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}