{"id":2975,"date":"2020-08-07T03:25:49","date_gmt":"2020-08-07T03:25:49","guid":{"rendered":"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/?page_id=2975"},"modified":"2020-08-07T03:30:59","modified_gmt":"2020-08-07T03:30:59","slug":"gimenez-clara","status":"publish","type":"page","link":"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/gimenez-clara\/","title":{"rendered":"Gimenez, Clara: Production of Kindlin-2 F0 Domain for Solid-State NMR Structural Biology"},"content":{"rendered":"<p><a href=\"https:\/\/rutgers.box.com\/s\/9667b8aolk5hp8apyjnaiwbd7cjotvoj\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-2827 alignleft\" src=\"http:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/08\/ClaraGimenezPoster__thumbnail-300x225.png\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/08\/ClaraGimenezPoster__thumbnail-300x225.png 300w, https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/08\/ClaraGimenezPoster__thumbnail-1024x768.png 1024w, https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/08\/ClaraGimenezPoster__thumbnail-768x576.png 768w, https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/wp-content\/uploads\/sites\/442\/2020\/08\/ClaraGimenezPoster__thumbnail.png 1067w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><br \/>\n<strong>Title:<\/strong> Production of Kindlin-2 F0 Domain for Solid-State NMR Structural Biology <\/p>\n<p><strong>Name:<\/strong> Clara Gimenez<\/p>\n<p><strong>Major:<\/strong> Biomedical Engineering<\/p>\n<p><strong>School affiliation:<\/strong> School of Engineering<\/p>\n<p><strong>Programs:<\/strong> Aresty Summer Science Program<\/p>\n<p><strong>Other contributors:<\/strong> Andrew J. Nieuwkoop and Jacqueline Perodeau<\/p>\n<p><strong>Abstract:<\/strong> Kindlin-2 (K2) is a protein known for mediating cellular chemotaxis by activating integrin. The mechanism of activation involves phosphatidyinositol phosphate (PIP) binding by both the F0 and PH domains (Fig. 1), but the role of each domain is unclear. Characterizing how each domain binds to its PIP ligand and interacts with the bulk membrane is the first step in unraveling the activation cascade. The Nieuwkoop laboratory focuses on applying solid-state nuclear magnetic resonance (ssNMR) techniques to the study of lipid bilayers and the protein that interact with them, including kindling-2.To study PIP binding, the PH and F0 domains were expressed via polymerase chain reaction (PCR) cloning process. Preliminary results found that by refining protocols high protein expression levels were yielded, setting the framework for future ssNMR experiments. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Title: Production of Kindlin-2 F0 Domain for Solid-State NMR Structural Biology Name: Clara Gimenez Major: Biomedical Engineering School affiliation: School of Engineering Programs: Aresty Summer Science Program Other contributors: Andrew &hellip; <a href=\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/gimenez-clara\/\" class=\"\">Read More<\/a><\/p>\n","protected":false},"author":970,"featured_media":0,"parent":441,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-custom.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-2975","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Gimenez, Clara: Production of Kindlin-2 F0 Domain for Solid-State NMR Structural Biology - Senior Exhibits<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.rutgers.edu\/nb-senior-exhibits\/partners\/aresty-research-virtual-symposium-2020\/gimenez-clara\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Gimenez, Clara: Production of Kindlin-2 F0 Domain for Solid-State NMR Structural Biology - Senior Exhibits\" \/>\n<meta property=\"og:description\" content=\"Title: Production of Kindlin-2 F0 Domain for Solid-State NMR Structural Biology Name: Clara Gimenez Major: Biomedical Engineering School affiliation: School of Engineering Programs: Aresty Summer Science Program Other contributors: Andrew &hellip; 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