{"id":529,"date":"2023-06-22T01:05:45","date_gmt":"2023-06-22T01:05:45","guid":{"rendered":"https:\/\/sites.rutgers.edu\/sahoo-lab\/?page_id=529"},"modified":"2023-07-19T00:53:11","modified_gmt":"2023-07-19T00:53:11","slug":"research-2","status":"publish","type":"page","link":"https:\/\/sites.rutgers.edu\/sahoo-lab\/site-styles-guidelines\/research-2\/","title":{"rendered":"Research"},"content":{"rendered":"<h3>Our lab believes in the philosophy of Bench-to-Bed side, where we strive to dig deep into the basic biological processes using various research models and apply the acquired knowledge to identify new drugs\/drug targets. In line with this philosophy, our lab has multiple projects at the interface of Basic &amp; Applied Biology, corroborated by our high impact publications and patents.<\/h3>\n<p>&nbsp;<\/p>\n<h5><strong>Model systems:<\/strong><\/h5>\n<ul>\n<li style=\"font-weight: 400\">Rodent models &#8211; primary neuron cultures, including dorsal root ganglions, cortical neurons, hippocampal neurons; sciatic nerve injury, spinal cord injury, optic nerve injury etc.<\/li>\n<li style=\"font-weight: 400\">Human iPSC-derived neurons for modeling various neurological diseases and understanding basic biological processes<\/li>\n<li><span style=\"font-weight: 400\">Cell lines \u2013 HEK293, HeLA, PC12, 3T3 etc.<\/span><\/li>\n<\/ul>\n<h5><\/h5>\n<h5><strong>Our Studies:<\/strong><\/h5>\n<p>&nbsp;<\/p>\n<div><b>Stress granules (SGs) in unstressed conditions<\/b><\/div>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-512 alignright\" src=\"http:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/06\/SGs-in-axons-300x300.jpg\" alt=\"\" width=\"300\" height=\"300\" srcset=\"https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/06\/SGs-in-axons-300x300.jpg 300w, https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/06\/SGs-in-axons-1024x1021.jpg 1024w, https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/06\/SGs-in-axons-150x150.jpg 150w, https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/06\/SGs-in-axons-768x765.jpg 768w, https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/06\/SGs-in-axons.jpg 1501w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<div class=\"page\" title=\"Page 1\">\n<div class=\"layoutArea\">\n<div class=\"column\">\n<p>&nbsp;<\/p>\n<p style=\"font-weight: 400\">Our studies focus on\u00a0how mRNAs are stored in axons and how they come out of the storage\u00a0depots in response to injury. We uncovered a previously unidentified role for SGs in axon\u00a0regeneration.<\/p>\n<p><span style=\"font-size: 1rem\"><span style=\"font-weight: 400\">Specifically, we showed that SG core\u00a0protein G3BP1 localizes to axons in the peripheral nervous system (PNS); axonal G3BP1aggregates into SG-like structures, and these\u00a0structures disassemble during axon regeneration. G3BP1 inhibits axonal protein synthesis and\u00a0reduces axon growth<\/span>.<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Axonal endogenous mechanism(s) to disassemble SGs<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-518 alignright\" src=\"http:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/06\/CK2-G3BP1-300x175.jpg\" alt=\"\" width=\"420\" height=\"245\" srcset=\"https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/06\/CK2-G3BP1-300x175.jpg 300w, https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/06\/CK2-G3BP1-1024x598.jpg 1024w, https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/06\/CK2-G3BP1-768x449.jpg 768w, https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/06\/CK2-G3BP1.jpg 1503w\" sizes=\"(max-width: 420px) 100vw, 420px\" \/><\/p>\n<p style=\"font-weight: 400\">Proteins synthesized locally in axons are needed for PNS axon regeneration. Several lines of evidence indicate that injured spinal cord axons have the ability to synthesize proteins when they are made competent to regenerate.<\/p>\n<p style=\"font-weight: 400\">Regenerating PNS axons have fewer G3BP1 granules than uninjured axons indicating that neurons have an endogenous mechanism(s) to disassemble axonal G3BP1 granules. Phosphorylation of the G3BP1 by Casein Kinase 2\u03b1 (CK2\u03b1) has been associated with SG disassembly in other cellular systems. Though CK2\u03b1 is known to be constitutively active, we showed that axonal CK2\u03b1 activity is temporally and spatially restricted by on-demand translation of axonal Csnk2a1 mRNA after injury. CK2\u03b1 appearance in axons after PNS nerve injury correlates with disassembly of axonal SGs and increased axon regeneration. Furthermore, we find that buffering the increased axoplasmic Ca\u00b2<sup>+<\/sup> after injury provides a means for the axonal translational machinery to specifically switch between synthesis of proteins needed for injury response to those that promote axon growth like CK2\u03b1.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Targeting Axonal SGs to accelerate nerve\u00a0<\/strong><b>regeneration<\/b><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-582 alignright\" src=\"http:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/07\/Screenshot-2023-07-17-at-3.23.24-PM-300x155.png\" alt=\"\" width=\"594\" height=\"307\" srcset=\"https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/07\/Screenshot-2023-07-17-at-3.23.24-PM-300x155.png 300w, https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/07\/Screenshot-2023-07-17-at-3.23.24-PM-1024x531.png 1024w, https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/07\/Screenshot-2023-07-17-at-3.23.24-PM-768x398.png 768w, https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/07\/Screenshot-2023-07-17-at-3.23.24-PM-1536x796.png 1536w, https:\/\/sites.rutgers.edu\/sahoo-lab\/wp-content\/uploads\/sites\/1040\/2023\/07\/Screenshot-2023-07-17-at-3.23.24-PM.png 1602w\" sizes=\"(max-width: 594px) 100vw, 594px\" \/><\/p>\n<p>Our data show that G3BP1 protein inhibits axonal protein synthesis, which in turn attenuates axon regeneration, and blocking its function accelerates axon growth. While working on this project, we discovered a cell permeable peptide derived from the G3BP1 protein that acts as a dominant negative factor for endogenous G3BP1. This <span style=\"font-size: 1rem\">peptide also accelerates both PNS and CNS axon <\/span><span style=\"font-size: 1rem\">regeneration\u00a0<\/span><span style=\"font-size: 1rem\">and prevents neurodegeneration (Sahoo et. al., Nat Comm, 2018, USA Patent # 10,668,128, USA <\/span><span style=\"font-size: 1rem\">Patent App # 16\/881,096). Our most recent data show that the same mechanism also works in CNS axon growth. <\/span><span style=\"font-size: 1rem\">.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Our lab believes in the philosophy of Bench-to-Bed side, where we strive to dig deep into the basic biological processes using various research models and apply the acquired knowledge to &hellip; <a href=\"https:\/\/sites.rutgers.edu\/sahoo-lab\/site-styles-guidelines\/research-2\/\" class=\"\">Read More<\/a><\/p>\n","protected":false},"author":2994,"featured_media":0,"parent":469,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-custom.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-529","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Research - Sahoo Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.rutgers.edu\/sahoo-lab\/site-styles-guidelines\/research-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Research - Sahoo Lab\" \/>\n<meta property=\"og:description\" content=\"Our lab believes in the philosophy of Bench-to-Bed side, where we strive to dig deep into the basic biological processes using various research models and apply the acquired knowledge to &hellip; 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