{"id":1128,"global_id":"sites.rutgers.edu\/shi-lab?id=1128","global_id_lineage":["sites.rutgers.edu\/shi-lab?id=1128"],"author":"790","status":"publish","date":"2022-07-07 01:37:30","date_utc":"2022-07-07 01:37:30","modified":"2022-09-01 18:48:49","modified_utc":"2022-09-01 18:48:49","url":"https:\/\/sites.rutgers.edu\/shi-lab\/event\/simon-scheuring-ccb-colloquium\/","rest_url":"https:\/\/sites.rutgers.edu\/shi-lab\/wp-json\/tribe\/events\/v1\/events\/1128","title":"CCB Colloquium – Professor Simon Scheuring, Weill Cornell Medicine,","description":"

\"Simon<\/strong><\/p>\n

\u201cBreaking Speed and Resolution Limitations of High-Speed AFM for Membrane Protein Structure-Function Analysis\u201d<\/strong><\/span><\/h1>\n

Simon Scheuring1,2,*
\n1 Weill Cornell Medicine, Department of Anesthesiology, 1300 York Avenue, New York, NY-10065, USA.
\n2 Weill Cornell Medicine, Department of Physiology and Biophysics, 1300 York Avenue, New York, NY-10065, USA<\/p>\n

High-speed atomic force microscopy (HS-AFM) is a powerful technique that provides dynamic movies of biomolecules at work [1]. We successfully used HS-AFM to take movies and determine dynamic parameters of membrane trafficking systems, transporters and channels.<\/p>\n

To break current temporal limitations to characterize molecular dynamics using HS-AFM, we developed HS-AFM line scanning (HS-AFM-LS) and HS-AFM height spectroscopy (HS-AFM-HS), methods whereby we scan the HS-AFM tip along a single scan line or keep it at a fixed position, respectively, and detect the motions of the molecules under the tip. This gives sub-nanometer spatial resolution combined with millisecond and microseconds temporal resolution of molecular fluctuations. HS-AFM-LS and HS-AFM-HS can be used in conjunction with HS-AFM imaging, thus giving access to a wide dynamic range [1]. This allowed us most recently to determine the single molecule kinetics of wild-type bacteriorhodopsin [2].<\/p>\n

To break current resolution limitations, we developed Localization AFM (LAFM). By applying localization image reconstruction algorithms to peak positions in high-speed AFM and conventional AFM data, we increase the resolution beyond the limits set by the tip radius and reach quasi-atomic resolution on soft protein surfaces in native and dynamic conditions. The LAFM method allows the calculation of high-resolution maps from either images of many molecules or many images of a single molecule acquired over time, opening new avenues for single molecule structural analysis [3].<\/p>\n

I will review these recent achievements, and present novel data and methods to integrate and strengthen HS-AFM as a powerful tool in structural biology and molecular biophysics. We reason that the progress of AFM must comprise two axes: First, making AFM\/HS-AFM a better tool. With the above-mentioned methods, we made significant progress in this axis over the last ~5 years. Second, making AFM\/HS-AFM data an integrated part of the structural biology \/ molecular biophysics toolbox. Such, we are developing now methods and data analysis and interpretation methods that interface with cryo-EM and X-ray crystallography, where AFM\/HS-AFM can contribute the urgently needed information about structure, dynamics and conformations under close-to-native conditions.<\/p>\n

References:<\/strong>
\n[1] Heath et al. Nature Communications, 2018, 9(1):4983, High-Speed AFM Height Spectroscopy (HS-AFM-HS): Microsecond dynamics of unlabeled biomolecules.
\n[2] Perrino et al., Nature Communications, 2021 12(7225), doi.org\/10.1038\/s41467-021-27580-2, Single molecule kinetics of bacteriorhodopsin by HS-AFM.
\n[3] Heath et al. Nature, 2021, 594(7863):385\u2013390, doi:10.1038\/s41586-021-03551-x, Localization Atomic Force Microscopy.<\/p>","excerpt":"","slug":"simon-scheuring-ccb-colloquium","image":false,"all_day":false,"start_date":"2022-10-18 08:00:00","start_date_details":{"year":"2022","month":"10","day":"18","hour":"08","minutes":"00","seconds":"00"},"end_date":"2022-10-18 17:00:00","end_date_details":{"year":"2022","month":"10","day":"18","hour":"17","minutes":"00","seconds":"00"},"utc_start_date":"2022-10-18 08:00:00","utc_start_date_details":{"year":"2022","month":"10","day":"18","hour":"08","minutes":"00","seconds":"00"},"utc_end_date":"2022-10-18 17:00:00","utc_end_date_details":{"year":"2022","month":"10","day":"18","hour":"17","minutes":"00","seconds":"00"},"timezone":"UTC+0","timezone_abbr":"UTC+0","cost":"","cost_details":{"currency_symbol":"","currency_code":"","currency_position":"prefix","values":[]},"website":"https:\/\/chem.rutgers.edu\/news-events\/jevents-list\/icalrepeat.detail\/2022\/10\/18\/356\/-\/professor-simon-scheuring-weill-cornell-medicine","show_map":true,"show_map_link":true,"hide_from_listings":false,"sticky":false,"featured":false,"categories":[],"tags":[],"venue":[],"organizer":[],"json_ld":{"@context":"http:\/\/schema.org","@type":"Event","name":"CCB Colloquium – Professor Simon Scheuring, Weill Cornell Medicine,","description":"<p>\u201cBreaking Speed and Resolution Limitations of High-Speed AFM for Membrane Protein Structure-Function Analysis\u201d Simon Scheuring1,2,* 1 Weill Cornell Medicine, Department of Anesthesiology, 1300 York Avenue, New York, NY-10065, USA. 2 … <a href="https:\/\/sites.rutgers.edu\/shi-lab\/event\/simon-scheuring-ccb-colloquium\/" class="">Read More<\/a><\/p>\\n","url":"https:\/\/sites.rutgers.edu\/shi-lab\/event\/simon-scheuring-ccb-colloquium\/","eventAttendanceMode":"https:\/\/schema.org\/OfflineEventAttendanceMode","eventStatus":"https:\/\/schema.org\/EventScheduled","startDate":"2022-10-18T08:00:00+00:00","endDate":"2022-10-18T17:00:00+00:00","performer":"Organization"}}