Terrestrial eDNA
We have pioneered new techniques to detect the presence of rare species living within terrestrial ecosystems. Based on the biology of the species of interest, different sampling methods target the DNA deposited on natural surfaces such as tree bark and leaves, or within the cambium.
Overall, most of these terrestrial techniques follow one simple principle —
Collect, Rinse, and Repeat!
Terrestrial
Using a damp, commercially available paint roller, we can pick up much of the genetic debris left behind on a surface by any recent visitors.
This technique has successfully detected species such as Spotted Lanternfly on deciduous trees, Coconut Rhinoceros Beetle on palm trees, and Little Brown Skink under cover boards.
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Taking advantage of the DNA deposited by species that tunnel or bore into trees, we can take a small core tissue sample and detect the presence of target species.
This technique has successfully detected Emerald Ash Borer.
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We can aggregate eDNA over large areas by washing the surface of foliage and collecting the DNA-laden drip water into buckets.
This technique has successfully detected species such as Spotted Lanternfly in vineyards and Brown Marmorated Stink Bugs in peach orchards.
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Many terrestrial organisms travel over or live within soil and mulch, continually depositing DNA.
By sampling soil or mulch, we can successfully detect pest species such as Coconut Rhinoceros Beetle, as well as a variety of forest mammals.
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We can integrate eDNA collection methods into existing agricultural operations to detect agricultural arthropod pests.
This technique has successfully detected Brown Marmorated Stink Bug on vegetable farms and peach orchards. Similar methods have detected Khapra Beetle within stored grain.
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