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Roller Aggregation

 

Commercial paint rollers can be used to collect eDNA from a variety of natural and artificial surfaces including trees, coverboards, and bridge supports. Below, we’ve supplemented our written protocol for sampling trees with a video demonstration. If any questions or concerns arise, please feel free to reach out to us here.

Protocol: Roller Aggregation Protocol


Required Sampling Supplies

 

1. Pressure Sprayer  2. Pump Charger  3. eDNA Filters (e.g. Smith-Root™ self desiccating)  
4. Gloves  5. Peristaltic pump  6.  Roller handles  7. Graduated cylinder  
8. Cleaned rollers in sterile bags 9.  Carboy filled with DI  10. Pump tubing  
11. Extension pole 12. Garbage bags  13. Extra sterile bags  
14. Writing utensils  15. Clipboard and datasheets

 


Video Demonstration

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