Roller Aggregation
Commercial paint rollers can be used to collect eDNA from a variety of natural and artificial surfaces including trees, coverboards, and bridge supports. Below, we’ve supplemented our written protocol for sampling trees with a video demonstration. If any questions or concerns arise, please feel free to reach out to us here.
Protocol: Roller Aggregation Protocol
Required Sampling Supplies
1. Pressure Sprayer 2. Pump Charger 3. eDNA Filters (e.g. Smith-Root™ self desiccating) 4. Gloves 5. Peristaltic pump 6. Roller handles 7. Graduated cylinder 8. Cleaned rollers in sterile bags 9. Carboy filled with DI 10. Pump tubing 11. Extension pole 12. Garbage bags 13. Extra sterile bags 14. Writing utensils 15. Clipboard and datasheets