Tree Coring
Endophagus insects, and perhaps other animals that live within living tree tissues, are thought to leave their DNA in or very near their tunnels and burrows via their chewing actions or their frass/excrement. We have preliminary evidence that this within-tissue DNA can be collected from tree cores, approximately 1 inch deep, and used to detect animals of interest (e.g., emerald ash borer). Below, you’ll find a written protocol for using tree cores to capture eDNA. If you have any questions, please feel free to reach out to us here.
Protocol: Tree Coring Protocol.docx
Image of Supplies
1. Gloves 2. Clipboard 3. Writing utensils 4. Datasheets 5. Increment hammer 6. Torch 7. 5 mL tubes and tube rack 8. 200 proof (100%) ethanol 9. 1.5 mL tubes and tube rack 10. Garbage bags