Publications
2022
Rapid detection of SARS-CoV-2 RNA in saliva via Cas13. S.S. Chandrasekaran*, S. Agrawal*, A. Fanton*, A.R. Jangid, B. Charrez, A.M. Escajeda, S. Son, R. McIntosh, H. Tran, A. Bhuiya, M.D. de León Derby, N.A. Switz, M. Armstrong, A.R. Harris, N. Prywes, M. Lukarska, S.B. Biering, D.C.J. Smock, A. Mok, G.J. Knott, Q. Dang, E. Van Dis, E. Dugan, S. Kim, T.Y. Liu; IGI Testing Consortium; Moehle EA, Kogut K, Eskenazi B, Harris E, Stanley SA, L.F. Lareau, M.X. Tan, D.A. Fletcher, J.A. Doudna, D.F. Savage, P.D. Hsu. Nat Biomed Eng. 6(8):944-956. PMID: 35953650. **Featured on the cover of Nature Biomedical Engineering. Innovative Genomics Institute press release: Link
2021
Sensitive and multiplexed RNA detection with Cas13 droplets and kinetic barcoding. S. Son, A. Lyden, J. Shu, S.I. Stephens, P. Fozouni, G.J. Knott, D.C.J. Smock, T.Y. Liu, D. Boehm, C. Simoneau, R. Kumar, J.A. Doudna, M. Ott, D.A. Fletcher. medRxiv [Preprint].
Accelerated RNA detection using tandem CRISPR nucleases. T.Y. Liu, G.J. Knott, D.C.J. Smock, J.J. Desmarais, S. Son, A. Bhuiya, S. Jakhanwal, N. Prywes, S. Agrawal, M. Díaz de León Derby, N.A. Switz, M. Armstrong, A.R. Harris, E.J. Charles, B.W. Thornton, P. Fozouni, J. Shu, S.I. Stephens, G.R. Kumar, C. Zhao, A. Mok, A.T. Iavarone, A.M. Escajeda, R. McIntosh, S. Kim, E.J. Dugan; IGI Testing Consortium, K.S. Pollard, M.X. Tan, M. Ott, D.A. Fletcher, L.F. Lareau, P.D. Hsu, D.F. Savage, J.A. Doudna. Nat Chem Biol. 17(9), 982–988. PMID: 34354262 **Featured on the cover of Nature Chemical Biology. UC Berkeley press release: Link
Rapid, point-of-care molecular diagnostics with Cas13. S. Agrawal*, A. Fanton*, S.S. Chandrasekaran*, B. Charrez, A.M. Escajeda, S. Son, R. Mcintosh, A. Bhuiya, M.D. de León Derby, N.A. Switz, M. Armstrong, A.R. Harris, N. Prywes, M. Lukarska, S.B. Biering, D.C.J. Smock, A. Mok, G.J. Knott, Q. Dang, E. Van Dis, E. Dugan, S. Kim, T.Y. Liu, IGI Testing Consortium, E. Harris, S.A. Stanley, L.F. Lareau, M.X. Tan, D.A. Fletcher, J.A. Doudna, D.F. Savage, P.D. Hsu. medRxiv [Preprint]. 2020.12.14.20247874
2020
Chemistry of Class 1 CRISPR-Cas effectors: binding, editing, and regulation. T.Y. Liu, J.A. Doudna. J. Biol. Chem. 295(42):14473-14487. PMID: 32817336
2019
Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble. T.Y. Liu*, J-J. Liu*, A.J. Aditham, E. Nogales, J.A. Doudna. Nature Communications. 10(1):3001. PMID: 31278272
2017
RNA and DNA targeting by a reconstituted Thermus thermophilus Type III-A CRISPR-Cas system. T.Y. Liu, A.T. Iavarone, J.A. Doudna. PLoS One. 12(1): e0170552. PMID: 28114398. Top 10% most cited articles of PLoS One in 2017.
Reconstitution of the tubular endoplasmic reticulum with purified components. R.E. Powers*, S.Y. Wang*, T.Y. Liu, T.A. Rapoport. Nature. 543(7644):257-260. PMID: 28225760
2015
Cis and trans interactions between atlastin molecules during membrane fusion. T.Y. Liu, X. Bian, F. Romano-Chernac, T. Shemesh, T.A. Rapoport, J. Hu. Proc. Natl. Acad. Sci. USA. 112(15):E1851-60. PMID: 25825753
2012
Lipid interaction of the C terminus and association of the transmembrane segments facilitate atlastin-mediated homotypic endoplasmic reticulum fusion. T.Y. Liu*, X. Bian*, S. Sun, X. Hu, R.W. Klemm, W.A. Prinz, T.A. Rapoport, J. Hu. Proc. Natl. Acad. Sci. U S A. 109(32): E2146-2154. PMID: 22802620
2011
Structures of the atlastin GTPase provide insight into homotypic fusion of endoplasmic reticulum membranes. X. Bian*, R.W. Klemm*, T.Y. Liu*, M. Zhang, S. Sun, X. Sui, X. Liu, T.A. Rapoport, J. Hu. Proc. Natl. Acad. Sci. U.S.A. 108(10): 3976-3981. PMID: 21368113
2010
Designed proteins to modulate cellular networks. A.L. Cortajarena*, T.Y. Liu*, M. Hochstrasser, L. Regan. ACS Chemical Biology. 5(6): 545-552. PMID: 20020775
Screening Libraries to identify proteins with desired binding activities using a split-GFP reassembly assay. M.E. Jackrel, A.L. Cortajarena, T.Y. Liu, L. Regan. ACS Chemical Biology. 5(6): 553-562. PMID: 20038141
2009
Chapter 3. Lymphotactin structural dynamics. B.F. Volkman, T.Y. Liu, F.C. Peterson. Methods in Enzymology. 461: 51-70. PMID: 19480914