Publications

2022

Rapid detection of SARS-CoV-2 RNA in saliva via Cas13. S.S. Chandrasekaran*, S. Agrawal*, A. Fanton*, A.R. Jangid, B. Charrez, A.M. Escajeda, S. Son, R. McIntosh, H. Tran, A. Bhuiya, M.D. de León Derby, N.A. Switz, M. Armstrong, A.R. Harris, N. Prywes, M. Lukarska, S.B. Biering, D.C.J. Smock, A. Mok, G.J. Knott, Q. Dang, E. Van Dis, E. Dugan, S. Kim, T.Y. Liu; IGI Testing Consortium; Moehle EA, Kogut K, Eskenazi B, Harris E, Stanley SA, L.F. Lareau, M.X. Tan, D.A. Fletcher, J.A. Doudna, D.F. Savage, P.D. Hsu. Nat Biomed Eng. 6(8):944-956. PMID: 35953650. **Featured on the cover of Nature Biomedical Engineering. Innovative Genomics Institute press release: Link

2021

Sensitive and multiplexed RNA detection with Cas13 droplets and kinetic barcoding. S. Son, A. Lyden, J. Shu, S.I. Stephens, P. Fozouni, G.J. Knott, D.C.J. Smock, T.Y. Liu, D. Boehm, C. Simoneau, R. Kumar, J.A. Doudna, M. Ott, D.A. Fletcher. medRxiv [Preprint].

Accelerated RNA detection using tandem CRISPR nucleases. T.Y. Liu, G.J. Knott, D.C.J. Smock, J.J. Desmarais, S. Son, A. Bhuiya, S. Jakhanwal, N. Prywes, S. Agrawal, M. Díaz de León Derby, N.A. Switz, M. Armstrong, A.R. Harris, E.J. Charles, B.W. Thornton, P. Fozouni, J. Shu, S.I. Stephens, G.R. Kumar, C. Zhao, A. Mok, A.T. Iavarone, A.M. Escajeda, R. McIntosh, S. Kim, E.J. Dugan; IGI Testing Consortium, K.S. Pollard, M.X. Tan, M. Ott, D.A. Fletcher, L.F. Lareau, P.D. Hsu, D.F. Savage, J.A. Doudna. Nat Chem Biol. 17(9), 982–988. PMID: 34354262   **Featured on the cover of Nature Chemical Biology. UC Berkeley press release: Link

Rapid, point-of-care molecular diagnostics with Cas13. S. Agrawal*, A. Fanton*, S.S. Chandrasekaran*, B. Charrez, A.M. Escajeda, S. Son, R. Mcintosh, A. Bhuiya, M.D. de León Derby, N.A. Switz, M. Armstrong, A.R. Harris, N. Prywes, M. Lukarska, S.B. Biering, D.C.J. Smock, A. Mok, G.J. Knott, Q. Dang, E. Van Dis, E. Dugan, S. Kim, T.Y. Liu, IGI Testing Consortium, E. Harris, S.A. Stanley, L.F. Lareau, M.X. Tan, D.A. Fletcher, J.A. Doudna, D.F. Savage, P.D. Hsu. medRxiv [Preprint]. 2020.12.14.20247874

2020

Chemistry of Class 1 CRISPR-Cas effectors: binding, editing, and regulation. T.Y. Liu, J.A. Doudna. J. Biol. Chem. 295(42):14473-14487. PMID: 32817336

2019

Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble. T.Y. Liu*, J-J. Liu*, A.J. Aditham, E. Nogales, J.A. Doudna. Nature Communications. 10(1):3001. PMID: 31278272

2017

RNA and DNA targeting by a reconstituted Thermus thermophilus Type III-A CRISPR-Cas system. T.Y. Liu, A.T. Iavarone, J.A. Doudna. PLoS One. 12(1): e0170552. PMID: 28114398. Top 10% most cited articles of PLoS One in 2017.

Reconstitution of the tubular endoplasmic reticulum with purified components. R.E. Powers*, S.Y. Wang*, T.Y. Liu, T.A. Rapoport. Nature. 543(7644):257-260. PMID: 28225760

2015

Cis and trans interactions between atlastin molecules during membrane fusion. T.Y. Liu, X. Bian, F. Romano-Chernac, T. Shemesh, T.A. Rapoport, J. Hu. Proc. Natl. Acad. Sci. USA. 112(15):E1851-60. PMID: 25825753

2012

Lipid interaction of the C terminus and association of the transmembrane segments facilitate atlastin-mediated homotypic endoplasmic reticulum fusion. T.Y. Liu*, X. Bian*, S. Sun, X. Hu, R.W. Klemm, W.A. Prinz, T.A. Rapoport, J. Hu. Proc. Natl. Acad. Sci. U S A. 109(32): E2146-2154. PMID: 22802620

2011

Structures of the atlastin GTPase provide insight into homotypic fusion of endoplasmic reticulum membranes. X. Bian*, R.W. Klemm*, T.Y. Liu*, M. Zhang, S. Sun, X. Sui, X. Liu, T.A. Rapoport, J. Hu. Proc. Natl. Acad. Sci. U.S.A. 108(10): 3976-3981. PMID: 21368113

2010

Designed proteins to modulate cellular networks. A.L. Cortajarena*, T.Y. Liu*, M. Hochstrasser, L. Regan. ACS Chemical Biology. 5(6): 545-552. PMID: 20020775

Screening Libraries to identify proteins with desired binding activities using a split-GFP reassembly assay. M.E. Jackrel, A.L. Cortajarena, T.Y. Liu, L. Regan. ACS Chemical Biology. 5(6): 553-562. PMID: 20038141

2009

Chapter 3. Lymphotactin structural dynamics. B.F. Volkman, T.Y. Liu, F.C. Peterson. Methods in Enzymology. 461: 51-70. PMID: 19480914