Dr. Yong Mao is currently an Associate Research Professor and the lead biologist in the Laboratory for Biomaterials Science. She joined Rutgers in 2013, having developed both academic and industrial research experience. Her research interests include cell-biomaterial interaction, extracellular matrix/synthetic hybrid bioactive scaffolds, stem cell technology, infection resistant biomaterials and wound healing. She has established and led multiple industrial collaborations. She also enjoys mentoring undergraduate students and developing their independent research capabilities. Dr. Mao directs the biological laboratory and microbiology laboratory at the LBR and leads the collaborations with internal and external research groups.
RESEARCH SUMMARY
Bioactive ECM-based polymeric scaffolds
Biodegradable polymeric scaffolds have gained tremendous interest in tissue regeneration in recent years due to their tunability in mechanical, structural and degradable properties. However, the lack of biological activity limits their integration with host tissues. To improve the biological activity of polymeric scaffolds for tissue-specific regeneration, we harness the biological cues from cell/tissue specific extracellular matrix (ECM) and combine them with polymeric scaffolds. By culturing lineage-specific cells on polymer scaffolds, followed by decellularization, hybrid scaffolds containing cell type specific ECM have been fabricated. These hybrid scaffolds guide the differentiation of human mesenchymal stem cells. Our research goal is to optimize the architecture of scaffolds, the biological activity of ECM and the presence of signaling peptides to support tissue regeneration.
In vitro expansion of primary cells for cell-based therapies
For many cell-based therapies, a large number of primary human cells are needed. For example, autologous chondrocyte implantation demands significant amplification of patients’ own chondrocytes to repair cartilage damage. However, the expansion of primary cells on tissue culture polystyrene results in dedifferentiation (loss of original cell phenotypes) of cells. We are exploring in vitro cell culture substrates to support cell expansion while minimizing cell dedifferentiation. Currently, modulating the cell adhesiveness to substrates and incorporating cell-type specific ECM components to substrates have demonstrated favorable effects on in vitro expansion of primary cells. We continue to explore the in vitro culturing conditions for human primary cells for therapeutic purposes.
Antimicrobial and anti-fibrotic activity of biological materials
Bacterial colonization in the wound environment or on implantable biomaterials leads to infection, which can result in more severe pathologies and failure of the implant. By characterizing the anti-bacterial activity of synthetic polymeric materials, we are able to facilitate the design and fabrication of infection-resistant biomaterials by chemists and material scientists.
Amniotic membranes (AM) have been used in wound treatment since ancient times. Recent years, processed AM have been developed into commercially available advanced biological wound dressing for treating chronic wounds. We are interested in understanding the properties and biological activities of AM. The antimicrobial peptides and exosomes (nano-sized vesicles) secreted by AM are our focuses in understanding the source and mechanism of antibacterial and anti-fibrotic activity of AM.
Publications
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Thymoquinone-Loaded Polymeric Films and Hydrogels for Bacterial Disinfection and Wound Healing. Biomedicines., Haq A, Kumar S, Mao Y, Berthiaume F, Michniak-Kohn B. , 2020
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600314/ -
In Vitro Evaluation of Recombinant Bone Morphogenetic Protein-2 Bioactivity for Regenerative Medicine., Fung SL, Wu X, Maceren JP, Mao Y, Kohn J., 2019
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761583/ -
Endogenous viable cells in lyopreserved amnion retain differentiation potential and anti-fibrotic activity in vitro. , Mao Y, Hoffman T, Dhall S, Singal A, Sathyamoorthy M, Danilkovitch A, Kohn J. , 2019
https://www.sciencedirect.com/science/article/pii/S174270611930412X -
Processed human amniotic fluid retains its antibacterial activity, Mao Y, Pierce J, Singh-Varma A, Boyer M, Kohn J, Reems JA. , 2019
https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-019-1812-8 -
Patterned electrical impulses delivered to Schwann cells by an artificial axon in the suspended-wire culture model, Merolli A, Mao Y, Voronin G, Steele J, Murthy NS, Kohn J, 2019
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404504/ -
Extracellular matrix derived from chondrocytes promotes rapid expansion of human primary chondrocytes in vitro with reduced dedifferentiation, Mao Y, Block T, Singh-Varma A, Sheldrake A, Leeth R, Griffey S and Kohn J. , 2019
https://pubmed.ncbi.nlm.nih.gov/30528605/ -
“Ruffled border” formation on a CaP-free substrate: a first step towards osteoclast-recruiting bone-grafts materials able to re-establish bone turn-over, Merolli A, Fung S, Murthy NS, Pashuck, ET, Mao Y, Wu X, Steele J, Martin D, Moghe P, Bromage T, Kohn J, 2018
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5862932/ -
The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated Pathogens., Mao Y, Singh-Varma A, Hoffman T, Dhall S, Danilkovitch A and Kohn J. , 2018
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872089/ -
An Innovative Laboratory Procedure to Expand Chondrocytes with Reduced Dedifferentiation. , Mao Y, Hoffman T, Wu A, Kohn J. , 2018
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5871131/ -
Influence of the three-dimensional culture of human bone marrow mesenchymal stromal cells within a macroporous polysaccharides scaffold on Pannexin 1 and Pannexin 3, Guerrero J, Oliveira H, Aid R, Bareille R, Siadous R, Letourneur D, Mao Y, Kohn J, Amédée J, 2018
https://pubmed.ncbi.nlm.nih.gov/29222846/ -
Enhancement of plasma protein adsorption and osteogenesis of hMSCs by functionalized siloxane coatings for titanium implants, Martínez-Ibáñez M, Murthy NS, Mao Y, Suay J, Gurruchaga M, Goñi I, Kohn J, 2018
https://pubmed.ncbi.nlm.nih.gov/28544508/ -
Antimicrobial Peptides Secreted From Human Cryopreserved Viable Amniotic Membrane Contribute to its Antibacterial Activity, Mao Y, Hoffman T, Singh-Varma A, Duan-Arnold Y, Moorman M, Danilkovitch A, Kohn J, 2017
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651856/ -
Cell type-specific extracellular matrix guided the differentiation of human mesenchymal stem cells in 3D polymeric scaffolds. , Mao Y, Hoffman T, Wu A, Goyal R, Kohn J., 2017
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5440495/ -
A suspended carbon fiber culture to model myelination by human Schwann cells, Merolli A, Mao Y, Kohn J. , 2017
https://pubmed.ncbi.nlm.nih.gov/28210970/ -
Optimization of Polymer-ECM Composite Scaffolds for Tissue Engineering: Effect of Cells and Culture Conditions on Polymeric Nanofiber Mats , Goyal R, Guvendiren M, Freeman O, Mao Y, Kohn J., 2017
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371874/